Ju Huiming, Zhang Jiaqing, Bai Lijing, Mu Yulian, Du Yutao, Yang Wenxian, Li Yong, Sheng Anzhi, Li Kui
1] Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China [2] College of Veterinary Medicine, Yangzhou University, Yangzhou 25009, Jiangsu, P. R. China.
1] Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China [2] Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, P. R. China.
Sci Rep. 2015 May 11;5:10152. doi: 10.1038/srep10152.
Sustained expression of the GH gene has been shown to have detrimental effects on the health of animals. In the current study, transgenic founder pigs, with controllable pig growth hormone (pGH) expression, were cloned via the handmade cloning method (HMC), and pGH expression levels were examined at the cellular and organismal levels. The serum pGH levels in 3 founder male pigs were found to be significantly higher after induction with intramuscular injection of doxycycline (DOX) compared to baseline. A daily dose of DOX was administered via feed to these animals for a period of 65 to 155 days. The growth rate, feed efficiency and pGH serum concentration increased in the DOX-induced transgenic group compared with the other groups. 8 numbers of animals were euthanized and the dressing percentage, loin muscle and lean meat percentage were significantly higher in the DOX-induced F1 transgenic group compared with the other groups. In this study a large population of transgenic pigs, with integrated controllable expression of a transgene, was obtained. The transgenic pigs were healthy and normal in terms of reproductive capability. At the same time, feed efficiency was improved, production processes were accelerated and meat yield was increased.
生长激素（GH）基因的持续表达已被证明对动物健康有不利影响。在本研究中，通过手工克隆方法（HMC）克隆了具有可控猪生长激素（pGH）表达的转基因创始猪，并在细胞和个体水平上检测了pGH表达水平。发现3只创始雄猪经肌肉注射强力霉素（DOX）诱导后，血清pGH水平显著高于基线水平。通过饲料向这些动物每日投喂一定剂量的DOX，持续65至155天。与其他组相比，DOX诱导的转基因组的生长速度、饲料效率和pGH血清浓度均有所提高。对8只动物实施安乐死后发现，DOX诱导的F1转基因组的屠宰率、腰大肌和瘦肉率均显著高于其他组。在本研究中，获得了大量整合有可控转基因表达的转基因猪。这些转基因猪在繁殖能力方面健康且正常。同时，饲料效率提高，生产过程加快，肉产量增加。
