Khurshid Adnan, Hassan Mukhtiar, Alam Muhammad Masroor, Aamir Uzma Bashir, Rehman Lubna, Sharif Salmaan, Shaukat Shahzad, Rana Muhammad Suleman, Angez Mehar, Zaidi Syed Sohail Zahoor
Department of Biochemistry, Hazara University, Mansehra, Pakistan.
Department of Virology, National Institute of Health, Islamabad, Pakistan.
J Clin Virol. 2015 Jun;67:25-30. doi: 10.1016/j.jcv.2015.03.021. Epub 2015 Mar 28.
Crimean Congo hemorrhagic fever (CCHF) has been reported from more than 30 countries in Africa, Asia, Eastern Europe and Middle East. The disease is considered endemic in Pakistan and neighboring countries like Iran and Afghanistan.
This study aimed to explore the genetic diversity of CCHF virus (CCHFV) detected in Pakistan and Afghanistan based on analysis of partial S-segment sequences.
During 2011, one hundred samples satisfying the CCHF case definition were tested by (ELISA) and RT-PCR for detection of IgM antibodies and viral RNA, respectively. Phylogenetic analysis was carried out on partial S-segment nucleotide sequences using MEGA 5.0.
Out of one hundred collected during 2011, 49 (49%) were positive for CCHF either by ELISA/RT-PCR or both. The mean age of the CCHFV positive cases was 30.32 years (range 18-56 years) and overall mortality rate was 20.4%. All CCHF virus isolates from this study clustered with strains previously reported from Pakistan, Iran and Afghanistan within the Asia-1 genogroup. Four distinct sub-clades were found circulating within Asia-1 genogroup. Six CCHFV strains found in Pakistan and Afghanistan grouped into a new sub-clade-D.
Data from this study shows that endemic foci of CCHFV span the international border between Pakistan and Afghanistan with genetically diverse variants circulating in this region. Our findings emphasize to establish a laboratory based surveillance program and devise health policy measures to control CCHF infection especially in Baluchistan.
非洲、亚洲、东欧和中东的30多个国家已报告发生克里米亚-刚果出血热（CCHF）。该病在巴基斯坦以及伊朗和阿富汗等邻国被视为地方病。
本研究旨在通过分析部分S片段序列，探索在巴基斯坦和阿富汗检测到的克里米亚-刚果出血热病毒（CCHFV）的遗传多样性。
2011年期间，对100份符合CCHF病例定义的样本分别进行酶联免疫吸附测定（ELISA）和逆转录-聚合酶链反应（RT-PCR），以检测IgM抗体和病毒RNA。使用MEGA 5.0对部分S片段核苷酸序列进行系统发育分析。
在2011年收集的100份样本中，49份（49%）通过ELISA/RT-PCR或两者检测均为CCHF阳性。CCHFV阳性病例的平均年龄为30.32岁（范围18 - 56岁），总死亡率为20.4%。本研究中的所有CCHF病毒分离株与之前在巴基斯坦、伊朗和阿富汗报告的毒株聚集在亚洲-1基因群内。在亚洲-1基因群内发现有四个不同的亚分支在传播。在巴基斯坦和阿富汗发现的6株CCHFV毒株归入一个新的亚分支-D。
本研究数据表明，CCHFV的地方病疫源地跨越巴基斯坦和阿富汗的国际边界，该地区有基因多样的变异株在传播。我们的研究结果强调要建立基于实验室的监测计划，并制定卫生政策措施来控制CCHF感染，特别是在俾路支省
