Buendía-Jiménez J A, Muñoz-Guzmán M A, Vega-López M A, Cuenca-Verde C, Martínez-Labat J P, Cuéllar-Ordaz J A, Alba-Hurtado F
Programa de Doctorado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México, Mexico.
Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico.
Vet Parasitol. 2015 Jun 30;211(1-2):60-6. doi: 10.1016/j.vetpar.2015.04.019. Epub 2015 Apr 24.
The abomasal expression of IL-2, IL-4, IL-6, IL-10 and IFNγ in lambs experimentally infected with Haemonchus contortus and its relationship to protection induced by a Taenia hydatigena larvae vesicular concentrate (ThLVC) were evaluated. The lambs that were only infected with H. contortus larvae showed a worm burden greater (p<0.05) than the lambs that received ThLVC prior to infection. Moreover, the lambs that received ThLVC showed a greater (p<0.05) number of blood eosinophils than the lambs that did not receive the ThLVC. In general, the lambs that received ThLVC prior to infection had a greater amount of eosinophils and mast cells and higher in situ expression of IFNγ, IL-2, IL-4, IL-6 and IL-10 in the abomasal wall than the lambs that were infected with H. contortus only or that received ThLVC (p<0.05) only. A higher expression of IL-2 and IFNγ in the submucosa compared to the abomasal mucosa and a higher expression of IL-4 in the abomasal mucosa compared to the submucosa was observed (p<0.05). These results suggest that there is a Th1 type response in the abomasal submucosa and a Th2 type response in in the abomasal mucosa. The amount of eosinophils and mast cells and the in situ expression of IFNγ, IL-2, IL-4 and IL-6 in the abomasal walls were negatively correlated with the worm burden (p<0.05). These results suggest that ThLVC is a non-specific immune stimulator for the abomasal immune response, and it is likely that the protection observed is the result of this effect.
评估了实验性感染捻转血矛线虫的羔羊皱胃中白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和干扰素γ（IFNγ）的表达及其与泡状带绦虫幼虫囊泡浓缩物（ThLVC）诱导的保护作用的关系。仅感染捻转血矛线虫幼虫的羔羊，其虫负荷比感染前接受ThLVC的羔羊更高（p<0.05）。此外，接受ThLVC的羔羊血液嗜酸性粒细胞数量比未接受ThLVC的羔羊更多（p<0.05）。总体而言，感染前接受ThLVC的羔羊皱胃壁中嗜酸性粒细胞和肥大细胞数量更多，IFNγ、IL-2、IL-4、IL-6和IL-10的原位表达高于仅感染捻转血矛线虫或仅接受ThLVC的羔羊（p<0.05）。与皱胃黏膜相比，黏膜下层中IL-2和IFNγ表达更高，与黏膜下层相比，皱胃黏膜中IL-4表达更高（p<0.05）。这些结果表明，皱胃黏膜下层存在Th1型反应，皱胃黏膜存在Th2型反应。皱胃壁中嗜酸性粒细胞和肥大细胞数量以及IFNγ、IL-2、IL-4和IL-6的原位表达与虫负荷呈负相关（p<0.05）。这些结果表明，ThLVC是皱胃免疫反应的非特异性免疫刺激剂，观察到的保护作用可能是这种效应的结果。
