Gorczyca Marcelina, Korchowiec Beata, Korchowiec Jacek, Trojan Sonia, Rubio-Magnieto Jenifer, Luis Santiago V, Rogalska Ewa
†Department of Theoretical Chemistry, Faculty of Chemistry, Jagiellonian University, ul. R. Ingardena 3, 30-060 Krakow, Poland.
‡Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, ul. R. Ingardena 3, 30-060 Krakow, Poland.
J Phys Chem B. 2015 Jun 4;119(22):6668-79. doi: 10.1021/acs.jpcb.5b02575. Epub 2015 May 20.
The interaction between five gemini amphiphilic pseudopeptides (GAPs) differing by the length of the central spacer and a model membrane lipid, 1,3-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol (cardiolipin) were studied with the aim to evaluate their possible antimicrobial properties. To this end, monomolecular films were formed at the air/water interface with pure cardiolipin or cardiolipin/GAPs mixtures; film properties were determined using surface pressure and surface potential measurements, as well as polarization-modulation infrared reflection-absorption spectroscopy. Moreover, to better understand the GAPs-phospholipid interaction at the molecular level, molecular dynamics simulations were performed. The results obtained indicate that the length of the central spacer has an effect on the interaction of GAPs with cardiolipin and on the properties of the lipid film. The GAPs with the longer linkers can be expected to be useful for biological membrane modification and for possible antimicrobial applications.
研究了五种中心间隔长度不同的双子型两亲性假肽（GAPs）与模型膜脂1,3-双[1,2-二肉豆蔻酰-sn-甘油-3-磷酸]-sn-甘油（心磷脂）之间的相互作用，旨在评估它们可能的抗菌性能。为此，在空气/水界面用纯心磷脂或心磷脂/GAPs混合物形成单分子膜；使用表面压力和表面电位测量以及偏振调制红外反射吸收光谱法测定膜的性质。此外，为了在分子水平上更好地理解GAPs与磷脂的相互作用，进行了分子动力学模拟。所得结果表明，中心间隔的长度对GAPs与心磷脂的相互作用以及脂膜的性质有影响。可以预期，具有较长连接基的GAPs可用于生物膜修饰和可能的抗菌应用。
