Wu Xiaoli, Ling Jing, Fu Ziyi, Ji Chenbo, Wu Jiangping, Xu Qing
Department of Women Health Care, Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, China.
Department of Obstetrics and Gynecology, Affiliated Jiangyin Hospital of South-East University, Jiangyin 214400, China.
Biomed Pharmacother. 2015 Apr;71:1-6. doi: 10.1016/j.biopha.2015.02.004. Epub 2015 Feb 18.
BACKGROUND/AIMS: Uterine leiomyoma is the ahead benign tumor of the female genital tract, which resulted in menstrual abnormalities, recurrent pregnancy loss, and other serious gynecological disorders in women. Recently, as the process of exploring the brief molecular mechanisms of tumorgenesis, microRNAs (miRNAs) have attracted much more attention.
In this study, we first confirmed that microRNA-197 (miR-197) was down-regulated significantly in human uterus leiomyoma by quantity real-time polymerase chain reaction, compared to normal uterus myometrium. Then we observed the potential effects of miR-197 overexpression on human uterus leiomyoma cells by cell counting kit 8, wound healing assay, and flow cytometric assessment separately.
The data showed that miR-197 could inhibit cell proliferation, induce cell apoptosis, and block cell migration in vitro. Coincidently, levonorgestrel (LNG), a well-known uterus leiomyoma therapy, could induce miR-197 expression in human uterus leiomyoma cells, and over-expression of miR-197 showed a synergy effect on human uterus leiomyoma cell proliferation and apoptosis with LNG.
In this study, the data showed that miR-197 could play an anti-oncogenic role in human uterus leiomyoma cells, and cooperate with LNG on the cell proliferation and apoptosis, which suggested that miR-197 might be a potential target and provided database for clinical treatment.
背景/目的：子宫平滑肌瘤是女性生殖道最常见的良性肿瘤，可导致女性月经异常、反复流产及其他严重的妇科疾病。近年来，随着对肿瘤发生分子机制的深入探索，微小RNA（miRNA）受到了更多关注。
在本研究中，我们首先通过定量实时聚合酶链反应证实，与正常子宫肌层相比，微小RNA-197（miR-197）在人子宫平滑肌瘤中显著下调。然后，我们分别通过细胞计数试剂盒8、伤口愈合试验和流式细胞术评估观察了miR-197过表达对人子宫平滑肌瘤细胞的潜在影响。
数据显示，miR-197在体外可抑制细胞增殖、诱导细胞凋亡并阻断细胞迁移。巧合的是，左炔诺孕酮（LNG）作为一种著名的子宫平滑肌瘤治疗药物，可诱导人子宫平滑肌瘤细胞中miR-197的表达，且miR-197的过表达在人子宫平滑肌瘤细胞增殖和凋亡方面与LNG表现出协同作用。
本研究数据表明，miR-197在人子宫平滑肌瘤细胞中可能发挥抑癌作用，并在细胞增殖和凋亡方面与LNG协同作用，这表明miR-197可能是一个潜在靶点，并为临床治疗提供了数据库。
