Moody M D, Joklik W K
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
Virology. 1989 Dec;173(2):437-46. doi: 10.1016/0042-6822(89)90556-4.
When cultured cells are injected with mixtures of cores of two reovirus strains, a high proportion of reassortants are monoreassortants, that is, virus particles that contain one genome segment of 1 parent and 9 genome segments of the other. We have isolated two complete sets of monoreassortants, those that contain a single serotype 2 genome segment and 9 serotype 3 genome segments, and those that contain 1 serotype 3 genome segment and 9 serotype 1 genome segments. We have used the former set of monoreassortants (because reovirus serotypes 2 and 3 are less closely related than serotypes 1 and 3) to assess the effect of all 10 genome segments, or rather of the proteins that they encode, in controlling parameters of the reovirus multiplication cycle such as yield size, extent of viral ssRNA, dsRNA and protein synthesis, plaque size, and cytopathogenicity. Among the major findings are: proteins lambda 2, mu 1/mu 1C, and sigma 3 control yield size and extent of RNA and protein synthesis; proteins mu 2 and sigma 1 control severity of cytopathic effects; and proteins sigma 1, mu 1/mu 1C, and mu 2 control plaque size. Identification of monoreassortant phenotypes is useful for identifying which viral proteins are functionally involved at the various stages of the reovirus multiplication cycle.
当向培养细胞中注射两种呼肠孤病毒株的核心混合物时，很大一部分重配体是单重配体，即含有一个亲本的一个基因组片段和另一个亲本的九个基因组片段的病毒颗粒。我们已经分离出了两组完整的单重配体，一组含有一个2型血清型基因组片段和九个3型血清型基因组片段，另一组含有一个3型血清型基因组片段和九个1型血清型基因组片段。我们使用了前一组单重配体（因为呼肠孤病毒2型和3型的亲缘关系不如1型和3型密切）来评估所有10个基因组片段，或者更确切地说是它们所编码的蛋白质，在控制呼肠孤病毒增殖周期参数方面的作用，如产量大小、病毒单链RNA、双链RNA和蛋白质合成的程度、噬斑大小和细胞致病性。主要发现包括：λ2、μ1/μ1C和σ3蛋白控制产量大小以及RNA和蛋白质合成的程度；μ2和σ1蛋白控制细胞病变效应的严重程度；σ1、μ1/μ1C和μ2蛋白控制噬斑大小。鉴定单重配体表型有助于确定在呼肠孤病毒增殖周期的各个阶段哪些病毒蛋白在功能上起作用。
