Kizling Michal, Draminska Sylwia, Stolarczyk Krzysztof, Tammela Petter, Wang Zhaohui, Nyholm Leif, Bilewicz Renata
Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
Nanotechnology and Functional Materials, Department of Engineering - The Ångström Laboratory, Uppsala University, Box 534, 751 21 Uppsala, Sweden.
Bioelectrochemistry. 2015 Dec;106(Pt A):34-40. doi: 10.1016/j.bioelechem.2015.04.012. Epub 2015 Apr 29.
A biofuel cell comprising electrodes based on supercapacitive materials - carbon nanotubes and nanocellulose/polypyrrole composite was utilized to power an oxygen biosensor. Laccase Trametes versicolor, immobilized on naphthylated multi walled carbon nanotubes, and fructose dehydrogenase, adsorbed on a porous polypyrrole matrix, were used as the cathode and anode bioelectrocatalysts, respectively. The nanomaterials employed as the supports for the enzymes increased the surface area of the electrodes and provide direct contact with the active sites of the enzymes. The anode modified with the conducting polymer layer exhibited significant pseudocapacitive properties providing superior performance also in the high energy mode, e.g., when switching on/off the powered device. Three air-fructose biofuel cells connected in a series converted chemical energy into electrical giving 2 mW power and open circuit potential of 2V. The biofuel cell system was tested under various externally applied resistances and used as a powering unit for a laboratory designed two-electrode minipotentiostat and a laccase based sensor for oxygen sensing. Best results in terms of long time measurement of oxygen levels were obtained in the pulse mode -45 s for measurement and 15 min for self-recharging of the powering unit.
一种基于超级电容材料（碳纳米管和纳米纤维素/聚吡咯复合材料）的生物燃料电池被用于为氧生物传感器供电。固定在萘化多壁碳纳米管上的云芝漆酶和吸附在多孔聚吡咯基质上的果糖脱氢酶分别用作阴极和阳极生物电催化剂。用作酶载体的纳米材料增加了电极的表面积，并提供了与酶活性位点的直接接触。用导电聚合物层修饰的阳极表现出显著的赝电容特性，在高能模式下（例如，在开启/关闭供电设备时）也具有优异的性能。三个串联连接的空气-果糖生物燃料电池将化学能转化为电能，产生2毫瓦的功率和2伏的开路电位。该生物燃料电池系统在各种外部施加的电阻下进行了测试，并用作实验室设计的双电极微型恒电位仪和基于漆酶的氧传感传感器的供电单元。在脉冲模式下获得了氧水平长时间测量的最佳结果——测量45秒，供电单元自充电15分钟。
