Mansour Lamjed, Harrath Abdel Halim, Abdel-Baki Abdel-Azeem S, Al-Quraishy Saleh, Al Suliman Y Omar
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Unite de Recherche de Biologie Integrative et Ecologie Evolutive et Fonctionnelle des Milieux Aquatiques, Departement de Biologie, Faculte des Sciences de Tunis, Universite de Tunis El Manar, Tunisia.
Folia Parasitol (Praha). 2015 Jan 1;62:2015.010. doi: 10.14411/fp.2015.010.
During a survey the occurrence of Kudoa quraishii Mansour, Harrath, Abd-Elkader, Alwasel, Abdel-Baki et Al Omar, 2014, recently identified in the muscles of the Indian mackerel, Rastrelliger kanagurta (Cuvier), a species of Kudoa Meglitsch, 1947 infecting oocytes of mature females of the same host fish was found. The new species, for which the name Kudoa saudiensis sp. n. is proposed, infects oocytes that are enlarged with a whitish colour. The parasite develops in vesicular polysporous plasmodia within the oocyte. Infection occurs with a mean prevalence of 20% (7/35) of examined females. Mature spores are quadratic in shape in apical view, having four equal valves and four symmetrical polar capsules. Fresh spores are 2.4-3.6 µm long (mean ± SD 3.1 ± 0.3 µm), 4.3-5.4 µm (4.7 ± 0.3 µm) wide and 3.4-4.3 µm (3.8 ± 0.3 µm) in thickness and long. The smaller size of the new Kudoa species was the distinctive feature that separates it from all previously described species. Molecular analysis based on the SSU rDNA sequences shows that the highest percentage of similarity of 98.5% was observed with K. ovivora Swearer et Robertson, 1999, reported from oocytes of labroid fish from the Caribbean coasts of Panama. The percentage of similarity was 98% with K. azevedoi Mansour, Thabet, Chourabi, Harrath, Gtari, Al Omar et Ben Hassine, 2013 and 89% with K. quraishii. Phylogenetic analysis of the SSU and LSU rDNA data revealed a consistent of the new species with K. azevedoi and K. ovivora. Our findings support the creation of Kudoa saudiensis sp. n. that infects oocytes of the Indian mackerel Rastrelliger kanagurta.
在一项调查中，发现了2014年由曼苏尔、哈拉特、阿卜杜勒 - 卡迪尔、阿尔瓦塞尔、阿卜杜勒 - 巴基和奥马尔鉴定的库多虫属物种库多·古莱什虫（Kudoa quraishii），该物种最近在印度鲭（Rastrelliger kanagurta，居维叶）的肌肉中被发现，印度鲭是1947年梅格利奇描述的库多虫属的一种宿主鱼类，该物种还感染同一宿主鱼类成熟雌性的卵母细胞。我们提出了一个新物种，命名为沙特库多虫（Kudoa saudiensis sp. n.），它感染的卵母细胞会肿大并呈白色。该寄生虫在卵母细胞内的泡状多孢子原质团中发育。在所检查的雌性中，感染的平均患病率为20%（7/35）。成熟孢子在顶面观呈方形，有四个相等的瓣和四个对称的极囊。新鲜孢子长2.4 - 3.6 µm（平均±标准差3.1±0.3 µm），宽4.3 - 5.4 µm（4.7±0.3 µm），厚3.4 - 4.3 µm（3.8±0.3 µm）。这种新库多虫物种较小的尺寸是将其与所有先前描述的物种区分开来的显著特征。基于小亚基核糖体DNA（SSU rDNA）序列的分子分析表明，与1999年从巴拿马加勒比海岸的隆头鱼科鱼类卵母细胞中报道的卵形库多虫（K. ovivora）相似度最高，为98.5%。与2013年的阿泽维多库多虫（K. azevedoi）相似度为98%，与库多·古莱什虫相似度为89%。对SSU和大亚基核糖体DNA（LSU rDNA）数据的系统发育分析表明，新物种与阿泽维多库多虫和卵形库多虫一致。我们的研究结果支持创建感染印度鲭卵母细胞的沙特库多虫这一新物种。
