Oleszko Adam, Olsztyńska-Janus Sylwia, Walski Tomasz, Grzeszczuk-Kuć Karolina, Bujok Jolanta, Gałecka Katarzyna, Czerski Albert, Witkiewicz Wojciech, Komorowska Małgorzata
Institute of Biomedical Engineering and Instrumentation, Faculty of Fundamental Problems of Technology, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland ; Regional Specialist Hospital in Wrocław, Research and Development Centre, Kamieńskiego 73a, 51-124 Wrocław, Poland.
Institute of Biomedical Engineering and Instrumentation, Faculty of Fundamental Problems of Technology, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
Biomed Res Int. 2015;2015:245607. doi: 10.1155/2015/245607. Epub 2015 Apr 19.
During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS) are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The main breakdown product of oxidized lipids is the malondialdehyde (MDA). A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS) method. Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance (FTIR-ATR) spectroscopy, which enables determination of primary peroxidation products. We examined the influence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods. Linear correlation between these methods was shown. The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep. A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.
在血液透析（HD）过程中，由于血液与透析器表面接触，免疫系统被激活，活性氧（ROS）被释放到血浆中。血浆中所含的脂质和蛋白质尤其容易受到ROS的影响，它们会发生过氧化反应。氧化脂质的主要分解产物是丙二醛（MDA）。测量MDA浓度的常用方法是硫代巴比妥酸反应性物质（TBARS）法。尽管在血液透析过程中血浆中会形成MDA，但其浓度会降低，因为它会在透析器中从血液中被清除。因此，本研究提出了傅里叶变换红外衰减全反射（FTIR-ATR）光谱法，该方法能够测定初级过氧化产物。我们使用TBARS和FTIR-ATR方法，研究了向先前从血浆样本中提取的脂质悬浮液中添加过氧化氢的量对脂质过氧化的影响。结果表明这两种方法之间存在线性相关性。所提出的方法在评估绵羊血液透析过程中血浆脂质过氧化程度的变化时是有效的。使用FTIR-ATR进行测量显示，治疗15分钟和240分钟后血浆脂质过氧化增加，而TBARS浓度则分别较低。
