Hakima Laleh, Adler Esther, Prystowsky Michael, Schiff Bradley, Schlecht Nicolas, Fox Amy, Suhrland Mark, Khader Samer N
Department of Pathology, Montefiore Medical Center, Bronx, New York.
Department of Otolaryngology, Head & Neck Surgery, Montefiore Medical Center, Bronx, New York.
Diagn Cytopathol. 2015 Sep;43(9):683-7. doi: 10.1002/dc.23278. Epub 2015 May 12.
Human papillomavirus (HPV) positive head and neck squamous cell carcinoma (HNSCC) accounts for 25% of HNSCCs and frequently presents with neck lymph node metastases. We investigated utilizing cytology needle rinse material for HPV DNA testing by Hybrid Capture 2 molecular testing (HC2) as an alternative to p16 immunohistochemistry.
Twenty-two cases of HNSCC presenting with neck lymph node metastasis were prospectively identified by assessment of Diff Quik stained cytology smears. An aliquot of the needle rinse material from the lymph node was analyzed for HPV status using standard HC2 protocol. P16 status was determined with immunohistochemistry on the cell block and/or surgically obtained tumor.
The mean age of patients with p16 negative HNSCC was 7 years older than p16 positive disease (Table ). Primary tumor subsites were as follows: 17 oropharynx, 1 hypophayrnx, 3 larynx, and 1 oral cavity (Table ). All ten p16 negative patients had a history of smoking compared with 33% of p16 positive. Only 3 (25%) of p16 positive tumors demonstrated keratinization, whereas 90% of the p16 negative tumors keratinized (Fig. 1). Twelve of 22 HNSCC cases (55%) were p16 positive, of which 7 (58%) tested positive for HPV by HC2. Ten cases (45%) were negative for p16, all of which were negative for HPV by HC2 (Table ).
Molecular testing for HPV using HC2 on needle rinse material of FNA of HNSCC is a useful method of determining HPV status in HNSCC.
人乳头瘤病毒（HPV）阳性的头颈部鳞状细胞癌（HNSCC）占HNSCC的25%，且常伴有颈部淋巴结转移。我们研究了利用细胞学针冲洗材料通过杂交捕获2分子检测（HC2）进行HPV DNA检测，作为p16免疫组化的替代方法。
通过评估Diff Quik染色的细胞学涂片，前瞻性地确定了22例伴有颈部淋巴结转移的HNSCC病例。使用标准HC2方案分析来自淋巴结的针冲洗材料的等分试样的HPV状态。通过对细胞块和/或手术获得的肿瘤进行免疫组化来确定p16状态。
p16阴性的HNSCC患者的平均年龄比p16阳性疾病患者大7岁（表）。原发肿瘤部位如下：17例口咽，1例下咽，3例喉，1例口腔（表）。所有10例p16阴性患者都有吸烟史，而p16阳性患者中有33%有吸烟史。p16阳性肿瘤中只有3例（25%）表现为角化，而p16阴性肿瘤中有90%表现为角化（图1）。22例HNSCC病例中有12例（55%）p16阳性，其中7例（58%）通过HC2检测HPV呈阳性。10例（45%）p16阴性，所有这些病例通过HC2检测HPV均为阴性（表）。
在HNSCC的细针穿刺抽吸（FNA）针冲洗材料上使用HC2对HPV进行分子检测是确定HNSCC中HPV状态的一种有用方法。
