Yamamoto Shuhei, Fei Jiahui, Okochi Mina, Shimizu Kazunori, Yusa Akiko, Kondo Naoto, Iwata Hiroji, Nakanishi Hayao, Honda Hiroyuki
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Nagoya, 464-8603, Japan.
Bioprocess Biosyst Eng. 2015 Sep;38(9):1693-704. doi: 10.1007/s00449-015-1412-9. Epub 2015 May 12.
Detecting and analyzing circulating tumor cells (CTCs) in the blood of cancer patients is a promising approach for the early diagnosis of metastasis. Previously, we developed a size-selective filter for capturing CTCs, but its use was time consuming, particularly for capturing CTCs from large volumes of blood. In the present study, we describe the use of a magnetic capture column for rapid and efficient isolation of CTCs, which were magnetically labeled with magnetite cationic liposomes. In the capturing process, large volumes of blood containing magnetically labeled cancer cells were introduced into the column at a high flow rate to capture the cells, which were then added into the filter at a low flow rate. Our results show that the combined use of the column and filter decreased the required time for the spiked cancer cell capture, and the recovery rate of the spiked cancer cells from blood was significantly higher using the combination process (80.7 %) than that using the filter alone (64.7 %). Moreover, almost twice the number of CTCs could be captured from the blood of metastatic model mice using the combination process. These results suggest that the developed process would be useful for the rapid and efficient isolation of CTCs.
检测和分析癌症患者血液中的循环肿瘤细胞（CTC）是一种很有前景的转移早期诊断方法。此前，我们开发了一种用于捕获CTC的尺寸选择性过滤器，但使用起来很耗时，尤其是从大量血液中捕获CTC时。在本研究中，我们描述了使用磁捕获柱快速高效分离CTC的方法，这些CTC用磁铁矿阳离子脂质体进行了磁性标记。在捕获过程中，将含有磁性标记癌细胞的大量血液以高流速引入柱中以捕获细胞，然后以低流速将其添加到过滤器中。我们的结果表明，柱和过滤器的联合使用减少了加标癌细胞捕获所需的时间，并且使用联合方法从血液中回收加标癌细胞的比率（80.7%）明显高于单独使用过滤器时（64.7%）。此外，使用联合方法从转移性模型小鼠的血液中捕获的CTC数量几乎是原来的两倍。这些结果表明，所开发的方法将有助于快速高效地分离CTC。
