Bohn Anja B, Brems-Eskildsen Anne S, Møller Bjarne K, Horsman Michael R
Department of Experimental Clinical Oncology, Aarhus University Hospital, Aarhus, Denmark Department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark
Department of Pathology, Aarhus University Hospital, Aarhus, Denmark.
Anticancer Res. 2015 May;35(5):2559-64.
BACKGROUND/AIM: Combretastatin A-4 phosphate (CA4P) is a vascular-disrupting agent which affects the level of circulating neutrophils. Since tumor-associated macrophages and neutrophils may collaborate, we compared the effect of CA4P treatment on monocytes/macrophages and neutrophils.
CDF1 mice with a C3H mammary carcinoma foot tumor were injected intraperitoneally with CA4P. Blood samples were taken and tumors excised at various time-points after treatment. Circulating monocytes and granulocytes were detected by flow cytometry and the tumor levels of these cell types was estimated immunohistochemically.
CA4P induced similar oscillating effects on the level of circulating monocytes and of neutrophils, with an initial decrease followed by an increase and a return to control levels at 6-h and 24-h, respectively. In tumors, only the macrophage level decreased significantly after treatment.
CA4P induced similar changes in the level of circulating monocytes and neutrophils, but only affected the fraction of macrophages significantly.
背景/目的：磷酸考布他汀A - 4（CA4P）是一种血管破坏剂，可影响循环中性粒细胞水平。鉴于肿瘤相关巨噬细胞和中性粒细胞可能存在协作关系，我们比较了CA4P治疗对单核细胞/巨噬细胞和中性粒细胞的影响。
给患有C3H乳腺足部肿瘤的CDF1小鼠腹腔注射CA4P。在治疗后的不同时间点采集血样并切除肿瘤。通过流式细胞术检测循环单核细胞和粒细胞，并通过免疫组织化学评估这些细胞类型在肿瘤中的水平。
CA4P对循环单核细胞和中性粒细胞水平产生相似的振荡效应，最初下降，随后分别在6小时和24小时时上升并恢复到对照水平。在肿瘤中，治疗后仅巨噬细胞水平显著下降。
CA4P在循环单核细胞和中性粒细胞水平上诱导了相似的变化，但仅显著影响巨噬细胞比例。
