Nan Wenbin, Liu Rui, Chen Hongli, Xu Zhihao, Chen Jiannan, Wang Manman, Yuan Zhiqing
Department of Life Sciences and Technology, Xinxiang Medical University, Henan, China.
Sanquan Medical College, Xinxiang Medical University, Henan, China.
Wounds. 2015 May;27(5):134-40.
The aim of this study was to examine the effects of human umbilical cord mesenchymal stem cells (hUCMSCs) in combination with a collagen-fibrin double-layered membrane on wound healing in mice. A collagen-fibrin double-layered membrane was prepared, and the surface properties of the support material were investigated using a scanning electron microscope. Twenty-four mice were prepared for use as full-thickness skin wound models and randomly divided into 3 groups: group A, a control group in which the wounds were bound using a conventional method; group B, a group treated with hUCMSCs combined with a collagen membrane; and group C, a group treated with hUCMSCs combined with a collagen-fibrin double-layered membrane. The postoperative concrescence of the wounds was observed daily to evaluate the effects of the different treatments. Scanning electron microscope observation showed the collagen-fibrin scaffolds exhibited a highly porous and interconnected structure, and wound healing in the double-layered membrane group was better than in groups A or B. Treatment with hUCMSCs combined with a collagen-fibrin double-layered membrane accelerated wound healing.
本研究的目的是探讨人脐带间充质干细胞（hUCMSCs）与胶原 - 纤维蛋白双层膜联合应用对小鼠伤口愈合的影响。制备了胶原 - 纤维蛋白双层膜，并使用扫描电子显微镜研究了支撑材料的表面特性。将24只小鼠制备成全层皮肤伤口模型，并随机分为3组：A组为对照组，采用传统方法包扎伤口；B组为用hUCMSCs联合胶原膜治疗的组；C组为用hUCMSCs联合胶原 - 纤维蛋白双层膜治疗的组。每天观察伤口的术后愈合情况，以评估不同治疗方法的效果。扫描电子显微镜观察显示，胶原 - 纤维蛋白支架呈现出高度多孔且相互连通的结构，双层膜组的伤口愈合情况优于A组或B组。用hUCMSCs联合胶原 - 纤维蛋白双层膜治疗可加速伤口愈合。
