绞股蓝酸性多糖的性质及抗氧化活性研究。
Characterization and antioxidant activities of acidic polysaccharides from Gynostemma pentaphyllum (Thunb.) Markino.
机构信息
School of Pharmeutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, China.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
出版信息
Carbohydr Polym. 2015;127:209-14. doi: 10.1016/j.carbpol.2015.03.069. Epub 2015 Mar 28.
Three acid polysaccharides obtained from Gynostemma pentaphyllum (Thunb.) Markino (named as GPA1, GPA2 and GPA3) using combination of water extraction, ion-exchange and gel permeation chromatography were subjected to composition analysis and valuated for the antioxidant activity. The sugar content of GPA1, GPA2 and GPA3 were 54.55%, 85.70% and 91.34%, respectively. Monosaccharide analysis showed that GPA1, GPA2 and GPA3 were all composed of Man, Rha, GlcA, GalA, Glc, Gal, Ara and Fuc, besides, GPA2 and GPA3 also contained Xyl. The molecular weight of GPA1, GPA2 and GPA3 were 19.6kDa, 10.6 kDa and 6.7 kDa, respectively. In vitro antioxidant assay, GPA1, GPA2 and GPA3 could scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical, chelate ferrous ion and reduce ferric ion. The antioxidant activities of GPA3 were stronger than those of GPA1 and GPA2, suggesting that GPA3 has significant potential as a natural antioxidant agent.
从绞股蓝（Thunb.）Markino（命名为 GPA1、GPA2 和 GPA3）中提取的三种酸性多糖，采用水提、离子交换和凝胶渗透色谱相结合的方法进行了成分分析，并对其抗氧化活性进行了评价。GPA1、GPA2 和 GPA3 的糖含量分别为 54.55%、85.70%和 91.34%。单糖分析表明，GPA1、GPA2 和 GPA3 均由 Man、Rha、GlcA、GalA、Glc、Gal、Ara 和 Fuc 组成，此外，GPA2 和 GPA3 还含有 Xyl。GPA1、GPA2 和 GPA3 的分子量分别为 19.6kDa、10.6 kDa 和 6.7 kDa。体外抗氧化试验表明，GPA1、GPA2 和 GPA3 均可清除 1,1-二苯基-2-苦基肼（DPPH）自由基和羟自由基，螯合亚铁离子并还原铁离子。GPA3 的抗氧化活性强于 GPA1 和 GPA2，表明 GPA3 具有作为天然抗氧化剂的巨大潜力。
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