Baraket G, Abdelkrim A B, Salhi-Hannachi A
Laboratory of Molecular Genetics, Immunology & Biotechnology, Faculty of Sciences, University Tunis El Manar, El Manar, Tunis, Tunisia.
Laboratory of Molecular Genetics, Immunology & Biotechnology, Faculty of Sciences, University Tunis El Manar, El Manar, Tunis, Tunisia
Genet Mol Res. 2015 Apr 22;14(2):3817-32. doi: 10.4238/2015.April.22.11.
Cytoplasmic chloroplast DNA was explored to establish genetic relationships among Ficus carica cultivars and elucidate the molecular evolution of the species. The results suggest the occurrence of haplotype and nucleotide diversity. Conserved group I intron sequence motifs were detected and showed a common secondary structure, despite the presence of some mutations on their sequences. The neighbor-joining dendrogram showed a continuous diversity that characterizes local resources. The maximum parsimony tree, with an RI index of 0.507, indicated minimal homoplasy within the data set. Furthermore, our results demonstrate that the trnL intron is the seat of numerous substitutions. Herein, new insight on the mechanism involved in the evolution of the trnL intron in the fig is presented. From the study, it appears that there is an explicit rejection of the null hypothesis in F. carica. A scenario of positive selection and recent expansion of F. carica genotypes across Tunisia seems to be retained.
研究探索了细胞质叶绿体DNA，以建立无花果品种间的遗传关系，并阐明该物种的分子进化。结果表明存在单倍型和核苷酸多样性。尽管序列上存在一些突变，但检测到了保守的I组内含子序列基序，并显示出共同的二级结构。邻接法树状图显示出一种连续的多样性，这是当地资源的特征。最大简约树的RI指数为0.507，表明数据集中的同塑性最小。此外，我们的结果表明trnL内含子是大量替代发生的位点。本文提出了对无花果trnL内含子进化机制的新见解。从研究中可以看出，在无花果中明显拒绝了零假设。似乎保留了突尼斯境内无花果基因型的正选择和近期扩张的情况。
