Liu J H, Zhong H J, Dang J, Peng L, Zhu Y S
College of Xinhua, Ningxia University, Yinchuan, China.
Key Laboratory of Fertility Preservation and Maintenance, Ningxia Medical University, Ministry of Education, Yinchuan, China.
Genet Mol Res. 2015 Apr 27;14(2):4041-50. doi: 10.4238/2015.April.27.19.
Previous studies suggested that dopamine receptors may be associated with drug dependence and impulsive behavior. In this study, we examined whether dopamine receptor D1 (DRD1) is associated with heroin dependence and the impulsive behavior in patients with heroin dependence. The participants included 367 patients with heroin dependence and 372 healthy controls from a Chinese Han population. We examined the potential association between heroin dependence and 8 single-nucleotide polymorphisms (rs686, rs4867798, rs1799914, rs4532, rs5326, rs265981, rs10078714, rs10078866) of DRD1, and the associations between single single-nucleotide polymorphism, haplotypes, and impulsive behavior. Compared with the healthy controls, heroin dependence patients showed a significantly lower frequency of GG homozygotes of rs5326 (P = 0.027), significantly lower frequency of the G allele of rs5326 (P = 0.007, odds ratio = 0.718, 95% confidence interval = 0.565-0.913), and higher frequency of the rs265981 G allele (P = 0.0002, odds ratio = 1.711, 95% confidence interval = 1.281-2.287). Furthermore, strong linkage disequilibrium was observed in 2 blocks (D' > 0.9). However, no association was observed between haplotypes and heroin dependence in the 2 blocks. This genetic behavior correlation study showed that the 2 single-nucleotide polymorphisms, rs5326 and rs265981, were not associated with the impulsive behavior in patients with heroin dependence. These findings indicate that DRD1 gene polymorphisms are related to heroin dependence in a Chinese Han population and may be informative for future genetic or biological studies on heroin dependence.
先前的研究表明，多巴胺受体可能与药物依赖和冲动行为有关。在本研究中，我们调查了多巴胺受体D1（DRD1）是否与海洛因依赖以及海洛因依赖患者的冲动行为有关。参与者包括367名中国汉族海洛因依赖患者和372名健康对照者。我们研究了海洛因依赖与DRD1的8个单核苷酸多态性（rs686、rs4867798、rs1799914、rs4532、rs5326、rs265981、rs10078714、rs10078866）之间的潜在关联，以及单个单核苷酸多态性、单倍型与冲动行为之间的关联。与健康对照者相比，海洛因依赖患者rs5326的GG纯合子频率显著降低（P = 0.027），rs5326的G等位基因频率显著降低（P = 0.007，优势比 = 0.718，95%置信区间 = 0.565 - 0.913），而rs265981的G等位基因频率更高（P = 0.0002，优势比 = 1.711，95%置信区间 = 1.281 - 2.287）。此外，在2个区域观察到强连锁不平衡（D' > 0.9）。然而，在这2个区域中未观察到单倍型与海洛因依赖之间的关联。这项遗传行为相关性研究表明，rs5326和rs265981这2个单核苷酸多态性与海洛因依赖患者的冲动行为无关。这些发现表明，DRD1基因多态性与中国汉族人群的海洛因依赖有关，可能为未来关于海洛因依赖的遗传或生物学研究提供信息。
