通过将内部RNA 3WJ角从60°调整到90°或108°构建RNA三角形、正方形、五边形的简单方法。

Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60° to 90° or 108°.

作者信息

Khisamutdinov Emil F, Bui My Nguyen Hoan, Jasinski Daniel, Zhao Zhengyi, Cui Zheng, Guo Peixuan

机构信息

Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY, 40536, USA,

出版信息

Methods Mol Biol. 2015;1316:181-93. doi: 10.1007/978-1-4939-2730-2_15.

DOI:10.1007/978-1-4939-2730-2_15

PMID:25967062

Abstract

Precise shape control of architectures at the nanometer scale is an intriguing but extremely challenging facet. RNA has recently emerged as a unique material and thermostable building block for use in nanoparticle construction. Here, we describe a simple method from design to synthesis of RNA triangle, square, and pentagon by stretching RNA 3WJ native angle from 60° to 90° and 108°, using the three-way junction (3WJ) of the pRNA from bacteriophage phi29 dsDNA packaging motor. These methods for the construction of elegant polygons can be applied to other RNA building blocks including the utilization and application of RNA 4-way, 5-way, and other multi-way junctions.

摘要

在纳米尺度上对结构进行精确的形状控制是一个引人入胜但极具挑战性的方面。RNA最近已成为用于纳米颗粒构建的独特材料和热稳定构建模块。在这里，我们描述了一种简单的方法，通过将噬菌体phi29双链DNA包装马达的pRNA的三向接头（3WJ）的RNA 3WJ天然角度从60°拉伸到90°和108°，从设计到合成RNA三角形、正方形和五边形。这些构建精美多边形的方法可应用于其他RNA构建模块，包括RNA四向、五向和其他多向接头的利用和应用。