Zhang Jinfeng, Zhang Jiye
Appl Opt. 2015 Feb 1;54(4):885-9. doi: 10.1364/AO.54.000885.
Image distortion seriously affects the accuracy in microscope image measurement. One source of such distortion is related to the tilting of the microscope stage during laser scanning, thereby resulting in various degrees of inclination angles. This paper describes a novel technique that improves the traditional laser triangulation method by using multiple parallel laser beams that can solve the inclination problem. Moreover, a multi-light-spot measurement device, based on the improved laser triangulation technique, is proposed that can accurately detect the degree and directions of the inclination angles in real time. Furthermore, experimental results generated from a prototype of this device show that the new measurement system can effectively detect small inclination angles at a precision up to ±0.5 μrad.
图像畸变严重影响显微镜图像测量的准确性。这种畸变的一个来源与激光扫描过程中显微镜载物台的倾斜有关,从而导致不同程度的倾斜角度。本文介绍了一种新颖的技术,该技术通过使用多个平行激光束改进传统的激光三角测量法,从而能够解决倾斜问题。此外,还提出了一种基于改进激光三角测量技术的多光斑测量装置,该装置能够实时准确地检测倾斜角度的大小和方向。此外,该装置原型产生的实验结果表明,新的测量系统能够有效检测小至±0.5 μrad精度的倾斜角度。