He Jinping, Miyazaki Jun, Wang Nan, Tsurui Hiromichi, Kobayashi Takayoshi
Opt Express. 2015 Apr 20;23(8):9762-71. doi: 10.1364/OE.23.009762.
Nonlinear photothermal microscopy is applied in the imaging of biological tissues stained with chlorophyll and hematoxylin. Experimental results show that this type of organic molecules, which absorb light but transform dominant part of the absorbed energy into heat, may be ideal probes for photothermal imaging without photochemical toxicity. Picosecond pump and probe pulses, with central wavelengths of 488 and 632 nm, respectively, are spectrally filtered from a compact supercontinuum fiber laser source. Based on the light source, a compact and sensitive super-resolution imaging system is constructed. Further more, the imaging system is much less affected by thermal blurring than photothermal microscopes with continuous-wave light sources. The spatial resolution of nonlinear photothermal microscopy is ~ 188 nm. It is ~ 23% higher than commonly utilized linear photothermal microscopy experimentally and ~43% than conventional optical microscopy theoretically. The nonlinear photothermal imaging technology can be used in the evaluation of biological tissues with high-resolution and contrast.
非线性光热显微镜被应用于对用叶绿素和苏木精染色的生物组织进行成像。实验结果表明，这类吸收光但将吸收能量的主要部分转化为热的有机分子，可能是用于光热成像且无光化学毒性的理想探针。分别具有中心波长488纳米和632纳米的皮秒泵浦脉冲和探测脉冲，是从一个紧凑的超连续光纤激光源中进行光谱滤波得到的。基于该光源，构建了一个紧凑且灵敏的超分辨率成像系统。此外，与具有连续波光源的光热显微镜相比，该成像系统受热模糊的影响要小得多。非线性光热显微镜的空间分辨率约为188纳米。实验上它比常用的线性光热显微镜高约23%，理论上比传统光学显微镜高约43%。非线性光热成像技术可用于对生物组织进行高分辨率和高对比度的评估。
