一种用于测定血管紧张素I转换酶抑制肽生物活性的简便反相高效液相色谱法
A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides.
作者信息
Wang Wei, Wang Nan, Zhang Yu, Cai Zheng, Chen Qihe, He Guoqing
机构信息
Institute of Quality Standards for Agriculture Products, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.
出版信息
ISRN Biotechnol. 2012 Oct 21;2013:453910. doi: 10.5402/2013/453910. eCollection 2013.
A convenient and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for angiotensin I-converting enzyme inhibitory peptides assay was described in this paper. The mobile phase consisted of 70% A (0.05% TFA and 0.05% triethylamine in water, pH = 2.9-3.3) and 30% B (100% acetonitrile) using an Isogradient program. The flow rate was 0.5 mL/min. The absorb wavelength was 226.5 nm; the column temperature was controlled at 25°C. This method for angiotensin I-converting enzyme inhibitory peptides assay was convenient for the Iso-gradient program. The accuracy of the RT-HPLC method was verified by analyzing ACE inhibitory activity of the hydrolysate peptides of silkworm pupae protein, and the results showed that the RT-HPLC method was available for exploring new source of angiotensin I-converting enzyme inhibitory peptides rapidly and veraciously.
本文描述了一种用于测定血管紧张素I转换酶抑制肽的简便、准确的反相高效液相色谱（RP-HPLC）方法。流动相由70%的A（0.05%三氟乙酸和0.05%三乙胺的水溶液，pH = 2.9 - 3.3）和30%的B（100%乙腈）组成，采用等度洗脱程序。流速为0.5 mL/min。吸收波长为226.5 nm；柱温控制在25°C。这种用于测定血管紧张素I转换酶抑制肽的方法采用等度洗脱程序很方便。通过分析蚕蛹蛋白水解肽的ACE抑制活性验证了RT-HPLC方法的准确性，结果表明RT-HPLC方法可用于快速、准确地探索血管紧张素I转换酶抑制肽的新来源。
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