Fujimoto Hitoshi, Tsuji Hiroyuki, Okubo Chigusa, Fukuda Ichiro, Nishino Tomoki, Lee K Monica, Renne Roger, Yoshimura Hiroyuki
Product and Science Division, R&D Group, Japan Tobacco Inc. , Kanagawa , Japan .
Inhal Toxicol. 2015 Mar;27(4):224-36. doi: 10.3109/08958378.2015.1027799. Epub 2015 May 13.
The heated cigarette (HC) generates mainstream smoke by vaporizing the components of the tobacco rod using a carbon heat source at the cigarette tip. Mainstream smoke of HC contains markedly less chemical constituents compared to combusted cigarettes. Mainstream smoke from HC was generated under Health Canada Intense regimen and its biological effects were compared to those of Reference (3R4F) cigarettes, using nose-only 5-week and 13-week inhalation studies. In the 13-week study, SD rats were necropsied following exposure to mainstream smoke from each cigarette at 200, 600 or 1000 µg wet total particulate matter/L for 1 h/day, 7 days/week or following a 13-week recovery period. Histopathological changes in the respiratory tract were significantly lesser in HC groups; e.g. respiratory epithelial hyperplasia in the nasal cavity and accumulation of pigmented macrophages in alveoli. After a 13-week recovery, the lesions were completely or partially regressed, except for accumulation of pigmented macrophages in alveoli, in both HC and 3R4F groups. In the 5-week study, SD rats were necropsied following exposure to mainstream smoke of either cigarette at 600 or 1000 µg/L for 1 h, two times/day (with 30 min interval), 7 days/week or following a 4-week recovery period. Bronchoalveolar lavage fluid (BALF) analysis of neutrophil percentages and enzyme levels like γ-GT, ALP and LDH indicated that pulmonary inflammation was significantly less in HC groups compared to 3R4F groups. In conclusion, HC demonstrated significantly lower biological effects compared to 3R4F, based on the BALF parameters and histopathology.
加热卷烟（HC）通过使用位于烟嘴处的碳热源使烟丝成分汽化来产生主流烟气。与燃烧型卷烟相比，HC的主流烟气中化学成分明显更少。HC的主流烟气是在加拿大卫生部的强化方案下产生的，并通过仅经鼻吸入的5周和13周研究，将其生物学效应与参比（3R4F）卷烟的生物学效应进行比较。在13周的研究中，将SD大鼠暴露于来自每种卷烟的主流烟气中，浓度为200、600或1000μg湿总颗粒物/升，每天1小时，每周7天，持续13周，之后进行尸检，或者在13周恢复期后进行尸检。HC组呼吸道的组织病理学变化明显较轻；例如，鼻腔呼吸上皮增生和肺泡中色素沉着巨噬细胞的积聚。13周恢复期后，除了肺泡中色素沉着巨噬细胞的积聚外，HC组和3R4F组的病变均完全或部分消退。在5周的研究中，将SD大鼠暴露于浓度为600或1000μg/升的任一种卷烟的主流烟气中，每天两次，每次1小时（间隔30分钟），每周7天，持续5周，之后进行尸检，或者在4周恢复期后进行尸检。支气管肺泡灌洗液（BALF）中中性粒细胞百分比以及γ-GT、ALP和LDH等酶水平的分析表明，与3R4F组相比，HC组的肺部炎症明显较轻。总之，基于BALF参数和组织病理学，HC显示出比3R4F明显更低的生物学效应。
