Qiu Tian, Teng Yincheng, Wang Yudong, Xu Liang
Reprod Fertil Dev. 2015 May 14. doi: 10.1071/RD14503.
The effects of adoptive transfer of transforming growth factor (TGF)-β1-induced regulatory T (Treg) cells in preventing spontaneous abortion in mice were investigated. CD4+CD25- cells were isolated from the spleens of pregnant CBA/J mice and induced into Treg cells positive for CD4, CD25 and forkhead box P3 (FOXP3) ex vivo using interleukin (IL)-2 and TGF-β1. CBA/J mice were mated with DBA/2J mice to establish a model of spontaneous abortion and, on the first day of pregnancy, mice were injected intravenously with 2 × 105 either freshly isolated Treg cells or those induced with TGF-β1. After 14 days, the surviving and reabsorbed fetuses in both groups were counted, and serum cytokine concentrations were measured by ELISA. Adoptive transfer of CD4+CD25+ or TGF-β1-induced Treg cells significantly reduced the fetal resorption rate, increased serum IL-10 and TGF-β1 concentrations and decreased interferon-γ levels. In conclusion, the results of the present study indicate that adoptive transfer of TGF-β1-induced Treg cells prevents spontaneous abortion in mice by increasing the secretion of T helper (Th) 2 cytokines and decreasing the secretion of Th1 cytokines.
研究了过继转移转化生长因子（TGF）-β1诱导的调节性T（Treg）细胞对预防小鼠自然流产的作用。从怀孕CBA/J小鼠的脾脏中分离出CD4+CD25-细胞，并在体外使用白细胞介素（IL）-2和TGF-β1将其诱导为CD4、CD25和叉头框P3（FOXP3）阳性的Treg细胞。将CBA/J小鼠与DBA/2J小鼠交配以建立自然流产模型，在怀孕的第一天，给小鼠静脉注射2×105个新鲜分离的Treg细胞或用TGF-β1诱导的Treg细胞。14天后，统计两组中存活和吸收的胎儿数量，并通过酶联免疫吸附测定法测量血清细胞因子浓度。过继转移CD4+CD25+或TGF-β1诱导的Treg细胞可显著降低胎儿吸收率，提高血清IL-10和TGF-β1浓度，并降低干扰素-γ水平。总之，本研究结果表明，过继转移TGF-β1诱导的Treg细胞可通过增加辅助性T（Th）2细胞因子的分泌和减少Th1细胞因子的分泌来预防小鼠自然流产。
