Shi Ping, Lu Zenghui, He Yuanchuan, Chen Shijiang, Qin Shaorong, Qing Yuling, Yan Jun
a Institute of Chinese Caterpillar Fungus, Chongqing Academy of Chinese Materia Medica , Chongqing , China.
b Chongqing Key Laboratory of Chinese Medicine Resources, Chongqing Academy of Chinese Materia Medica , Chongqing , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4205-4206. doi: 10.3109/19401736.2015.1022741. Epub 2015 May 13.
The complete mitochondrial genome sequence of Hepialus gonggaensis was sequenced for the first time. The complete mtDNA sequence was 15,940 bp in length and contained 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and an AT-rich region, the gene composition and the arrangement of which were identical to other insects of Hepialidae. The overall base composition of the heavy strand was 41.14% A, 40.24% T, 11.17% C, and 7.45% G, with an AT content of 81.37%. The necleotide sequence data of 13 protein-coding genes of H. gonggaensis and other 10 Lepidoptera species were used for constructing the phylogenetic tree. It revealed that H. gonggaensis and other four Hepialidae species were clustered to a clade with high bootstraps values.
首次对贡嘎蝠蛾的完整线粒体基因组序列进行了测序。完整的线粒体DNA序列长度为15940 bp,包含13个蛋白质编码基因、两个rRNA基因、22个tRNA基因和一个富含AT的区域,其基因组成和排列与蝙蝠蛾科的其他昆虫相同。重链的总体碱基组成为A占41.14%、T占40.24%、C占11.17%、G占7.45%,AT含量为81.37%。利用贡嘎蝠蛾13个蛋白质编码基因的核苷酸序列数据以及其他10种鳞翅目物种的数据构建了系统发育树。结果表明,贡嘎蝠蛾和其他四种蝙蝠蛾科物种聚为一个分支,自展值较高。