Yashiro Masateru, Mii Sumiyuki, Aki Ryoichi, Hamada Yuko, Arakawa Nobuko, Kawahara Katsumasa, Hoffman Robert M, Amoh Yasuyuki
a Department of Dermatology ; Kitasato University School of Medicine ; Sagamihara , Japan.
Cell Cycle. 2015;14(14):2362-6. doi: 10.1080/15384101.2015.1042633. Epub 2015 May 13.
We have previously demonstrated that the neural stem-cell marker nestin is expressed in hair follicle stem cells located in the bulge area which are termed hair-follicle-associated pluripotent (HAP) stem cells. HAP stem cells from mouse and human could form spheres in culture, termed hair spheres, which are keratin 15-negative and CD34-positive and could differentiate to neurons, glia, keratinocytes, smooth muscle cells, and melanocytes in vitro. Subsequently, we demonstrated that nestin-expressing stem cells could effect nerve and spinal cord regeneration in mouse models. In the present study, we demonstrated that HAP stem cells differentiated to beating cardiac muscle cells. We separated the mouse vibrissa hair follicle into 3 parts (upper, middle, and lower), and suspended each part separately in DMEM containing 10% FBS. All three parts of hair follicle differentiated to beating cardiac muscle cells as well as neurons, glial cells, keratinocytes and smooth muscle cells. The differentiation potential to cardiac muscle is greatest in the upper part of the follicle. The beat rate of the cardiac muscle cells was stimulated by isoproterenol and inhibited by propanolol. HAP stem cells have potential for regenerative medicine for heart disease as well as nerve and spinal cord repair.
我们之前已经证明，神经干细胞标志物巢蛋白在位于毛囊隆突区的毛囊干细胞中表达，这些细胞被称为毛囊相关多能（HAP）干细胞。来自小鼠和人类的HAP干细胞在培养中可形成球体，称为毛球，其角蛋白15阴性、CD34阳性，并且在体外可分化为神经元、神经胶质细胞、角质形成细胞、平滑肌细胞和黑素细胞。随后，我们证明表达巢蛋白的干细胞可在小鼠模型中促进神经和脊髓再生。在本研究中，我们证明HAP干细胞可分化为跳动的心肌细胞。我们将小鼠触须毛囊分为3部分（上部、中部和下部），并将每部分分别悬浮于含10%胎牛血清的DMEM中。毛囊的所有三个部分均可分化为跳动的心肌细胞以及神经元、神经胶质细胞、角质形成细胞和平滑肌细胞。毛囊上部向心肌的分化潜能最大。心肌细胞的搏动频率受异丙肾上腺素刺激，并被普萘洛尔抑制。HAP干细胞在心脏病再生医学以及神经和脊髓修复方面具有潜力。
