铜绿假单胞菌61-3中与两种聚羟基脂肪酸酯颗粒结合的蛋白质的表征与鉴定
Characterization and identification of the proteins bound to two types of polyhydroxyalkanoate granules in Pseudomonas sp. 61-3.
作者信息
Hokamura Ayaka, Fujino Kanako, Isoda Yoshiko, Arizono Koji, Shiratsuchi Hideki, Matsusaki Hiromi
机构信息
a Faculty of Environmental and Symbiotic Sciences, Department of Food and Health Sciences , Prefectural University of Kumamoto , Kumamoto , Japan.
出版信息
Biosci Biotechnol Biochem. 2015;79(8):1369-77. doi: 10.1080/09168451.2015.1023250. Epub 2015 May 14.
Pseudomonas sp. 61-3 accumulates two types of polyhydroxyalkanoates (PHAs), poly(3-hydroxybutyrate) [P(3HB)], and poly(3HB-co-3-hydroxyalkanoates) [P(3HB-co-3HA)], and some proteins associated with their PHA granules have been identified. To date, PhaFPs (GA36) and PhaIPs (GA18) were identified from P(3HB-co-3HA) granules. In this study, the gene encoding GA24 associated with P(3HB) granule was identified as phbPPs. PhbPPs was composed of 192 amino acids with a calculated molecular mass of 20.4 kDa and was assumed to be a phasin. phbFPs gene and unknown ORF were also found on phb locus. PhbFPs was anticipated to be the transcriptional repressor of phbPPs gene. PhbPPs was bound to the P(3HB-co-3HA) granules with 3HB composition of more than 87 mol%, and PhaIPs and PhaFPs were bound to the P(3HB-co-3HA) granules with 3HA (C6-C12) composition of more than 13 mol% in the producing cells, suggesting that localization of these proteins is attributed to the monomer compositions of the copolymers.
假单胞菌属61 - 3积累两种类型的聚羟基脂肪酸酯（PHA），即聚（3 - 羟基丁酸酯）[P(3HB)]和聚（3 - 羟基丁酸酯 - co - 3 - 羟基脂肪酸酯）[P(3HB - co - 3HA)]，并且一些与其PHA颗粒相关的蛋白质已被鉴定出来。迄今为止，已从P(3HB - co - 3HA)颗粒中鉴定出PhaFPs（GA36）和PhaIPs（GA18）。在本研究中，与P(3HB)颗粒相关的编码GA24的基因被鉴定为phbPPs。PhbPPs由192个氨基酸组成，计算分子量为20.4 kDa，被认为是一种phasins蛋白。在phb基因座上还发现了phbFPs基因和未知的开放阅读框（ORF）。预计PhbFPs是phbPPs基因的转录阻遏物。在产生菌细胞中，PhbPPs与3HB组成超过87摩尔%的P(3HB - co - 3HA)颗粒结合，而PhaIPs和PhaFPs与3HA（C6 - C12）组成超过13摩尔%的P(3HB - co - 3HA)颗粒结合，这表明这些蛋白质的定位归因于共聚物的单体组成。
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