Abdoli Amir, Arbabi Mohsen, Dalimi Abdolhossein, Pirestani Majid
a Department of Parasitology, Faculty of Medical Sciences , Kashan University of Medical Science , Kashan , Iran.
Avian Pathol. 2015;44(4):319-22. doi: 10.1080/03079457.2015.1050583.
Neospora caninum is an intracellular protozoan parasite with a wide range of intermediate bird hosts. There is little information describing the prevalence and genetic characterization of N. caninum in bird hosts worldwide and in Iran. In this study, a total of 217 brain samples of house sparrow (Passer domesticus) were examined for N. caninum presence by nested polymerase chain reaction targeting the Nc-5 gene. N. caninum DNA was detected in 3.68% (8/217) of sparrows. Sequencing of the Nc5 genomic DNA revealed 97-99% of similarity with N. caninum sequences deposited in Genbank. To our knowledge, this study is the first molecular evidence of N. caninum DNA in bird hosts in Iran. The results of this study highlight the role of the house sparrow (Passer domesticus) in maintaining and spreading N. caninum infection to canines in the feral and domestic environment.
犬新孢子虫是一种细胞内原生动物寄生虫，有多种中间鸟类宿主。关于全球和伊朗鸟类宿主中犬新孢子虫的流行情况和基因特征的信息很少。在本研究中，通过针对Nc-5基因的巢式聚合酶链反应，对总共217份家麻雀（Passer domesticus）的脑样本进行了犬新孢子虫检测。在3.68%（8/217）的麻雀中检测到犬新孢子虫DNA。Nc5基因组DNA测序显示与Genbank中保存的犬新孢子虫序列有97-99%的相似性。据我们所知，本研究是伊朗鸟类宿主中存在犬新孢子虫DNA的首个分子证据。本研究结果突出了家麻雀（Passer domesticus）在维持犬新孢子虫感染并将其传播到野生和家养环境中的犬类方面的作用。
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