水豚中新孢子虫DNA的检测及系统发育分析

Detection of Neospora caninum DNA in capybaras and phylogenetic analysis.

作者信息

Truppel Jessé Henrique, Montiani-Ferreira Fabiano, Lange Rogério Ribas, Vilani Ricardo Guilherme D'Otaviano de Castro, Reifur Larissa, Boerger Walter, da Costa-Ribeiro Magda Clara Vieira, Thomaz-Soccol Vanete

机构信息

Master of Science in Parasitology, Federal University of Parana (UFPR), Curitiba, Parana, Brazil.

出版信息

Parasitol Int. 2010 Sep;59(3):376-9. doi: 10.1016/j.parint.2010.05.001. Epub 2010 May 11.

DOI:10.1016/j.parint.2010.05.001

PMID:20470895

Abstract

The role of rodents in the sylvatic cycle of Neospora sp. and in the neosporosis epidemiology is still uncertain. The aim of the present work was to detect Neospora caninum and to determine its prevalence in capybaras (Hydrochaeris hydrochaeris), to help elucidate the role of this rodent in the life cycle of the parasite. N.caninum DNA was detected by PCR using 4 different sets of primers specific to the Nc5 and ITS1 sequences. The parasite was found in the lymph nodes, heart, liver, and blood of 23% of the twenty-six capybaras studied. Sequencing the amplified DNA revealed 98% of similarity with N. caninum sequences deposited in the Genbank. Our findings provide the first molecular evidence of N. caninum infection in capybaras, supporting the hypothesis that these rodents can act as reservoirs of N. caninum and play a role in the life cycle of this parasite.

摘要

啮齿动物在新孢子虫属的野生动物循环以及新孢子虫病流行病学中的作用仍不明确。本研究的目的是检测犬新孢子虫并确定其在水豚（Hydrochaeris hydrochaeris）中的流行率，以帮助阐明这种啮齿动物在该寄生虫生命周期中的作用。使用4组针对Nc5和ITS1序列的不同引物，通过PCR检测犬新孢子虫DNA。在所研究的26只水豚中，23%的水豚的淋巴结、心脏、肝脏和血液中发现了该寄生虫。对扩增的DNA进行测序后发现，其与Genbank中登记的犬新孢子虫序列有98%的相似性。我们的研究结果提供了水豚感染犬新孢子虫的首个分子证据，支持了这些啮齿动物可作为犬新孢子虫储存宿主并在该寄生虫生命周期中发挥作用这一假说。