Camejo G, Linden T, Olsson U, Wiklund O, Lopez F, Bondjers G
Instituto Venezolano de Investigaciones Científicas, Centro de Biofísica y Bioquímica, Caracas, Venezuela.
Atherosclerosis. 1989 Oct;79(2-3):121-8. doi: 10.1016/0021-9150(89)90116-0.
The interactions of LDL with extracellular matrix proteoglycans apparently contribute to the accumulation of apo B-lipoproteins in atherogenesis. Serum LDL forms insoluble complexes with human arterial chondroitin sulfate proteoglycans (CSPG). While the amount of insolubilized LDL varies, serum from survivors of myocardial infarcts and ischaemic subjects shows higher values of CSPG-insolubilized LDL than serum from controls. In this study, we explored the relationship between the formation of LDL-CSPG complexes in serum and some LDL properties, using binding isotherms and characterization of isolated LDL from 12 healthy controls and 12 young myocardial infarct survivors. The amount of LDL insolubilized in serum from solutions of CSPG was found to be a function of the product Bt (total binding) x the amount of serum LDL-cholesterol. Furthermore, the Bt values for the isolated LDL from controls and patients could be predicted with more than 70% certainty by using a multiple regression model which included the cholesterol/protein ratio, protein/triglyceride ratio, isoelectric point and the affinity coefficient of the lipoprotein for CSPG. The results indicate that LDL-CSPG measurements in serum are dependent upon both LDL concentration and structural properties which are related to its tendency to form complexes with arterial CSPG.
低密度脂蛋白（LDL）与细胞外基质蛋白聚糖的相互作用显然在动脉粥样硬化形成过程中促成了载脂蛋白B-脂蛋白的积聚。血清LDL与人动脉硫酸软骨素蛋白聚糖（CSPG）形成不溶性复合物。虽然不溶性LDL的量有所不同，但心肌梗死幸存者和缺血受试者的血清中，CSPG不溶性LDL的值高于对照组血清。在本研究中，我们利用结合等温线以及对12名健康对照者和12名年轻心肌梗死幸存者分离出的LDL进行表征，探讨了血清中LDL-CSPG复合物的形成与LDL某些特性之间的关系。发现从CSPG溶液中血清中不溶性LDL的量是产物Bt（总结合量）乘以血清LDL-胆固醇量的函数。此外，通过使用包含胆固醇/蛋白质比率、蛋白质/甘油三酯比率、等电点以及脂蛋白对CSPG的亲和系数的多元回归模型，可以70%以上的确定性预测对照者和患者分离出的LDL的Bt值。结果表明，血清中LDL-CSPG的测量既取决于LDL浓度，也取决于与其与动脉CSPG形成复合物倾向相关的结构特性。
