Ammar Megahed H, Alghamdi Salem S, Migdadi Hussein M, Khan Muhammad A, El-Harty Ehab H, Al-Faifi Sulieman A
Legume Research Group, Plant Production Department, Faculty of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2015 May;22(3):340-50. doi: 10.1016/j.sjbs.2015.02.005. Epub 2015 Feb 16.
Forty faba bean (Vicia faba L.) genotypes were evaluated for their agro-morphological performance and molecular diversity under Central Region of Saudi Arabia conditions during 2010-11 and 2011-12 seasons. Field performance results showed that faba genotypes exhibited a significant amount of variation for their agro-morphological studied parameters. Giza40 recorded the tallest genotype (139.5 cm), highest number of seeds per plants (100.8), and the highest seed yield per plant (70.8 g). The best performing genotypes were Giza40, FLIP03-014FB, Gazira1 and Goff1. Genetic variability among genotypes was determined using Sequence Related Amplified Polymorphism (SRAP) and Amplified Fragment Length Polymorphism (AFLP) markers. A total of 183 amplified fragments (alleles) and 1758 polymorphic fragments (bands) in SRAP and 202 alleles and 716 bands in AFLP were obtained using six SRAP and four AFLP primer combinations respectively. Polymorphism information content (PIC) values for AFLP and SRAP markers were higher than 0.8, indicating the existence of a considerable amount of genetic diversity among faba tested genotypes. The UPGMA based clustering of faba genotypes was largely based on origin and/or genetic background. Result of cluster analysis based on SRAP showed weak and not significant correlation while, it was highly significant based on AFLP analysis with agro-morphological characters (r = 0.01, p > 0.54 and r = 0.26, p < 0.004 respectively). Combined SRAP and AFLP markers proved to be significantly useful for genetic diversity assessment at molecular level. They exhibited high discrimination power, and were able to distinguish the faba bean genotypes with high efficiency and accuracy levels.
2010 - 11年和2011 - 12年期间，在沙特阿拉伯中部地区的条件下，对40个蚕豆（Vicia faba L.）基因型的农艺形态表现和分子多样性进行了评估。田间表现结果表明，蚕豆基因型在所研究的农艺形态参数上表现出显著的变异。吉萨40号表现出最高的基因型（139.5厘米）、单株最高种子数（100.8粒）和单株最高种子产量（70.8克）。表现最佳的基因型是吉萨40号、FLIP03 - 014FB、加济拉1号和戈夫1号。利用序列相关扩增多态性（SRAP）和扩增片段长度多态性（AFLP）标记确定了基因型之间的遗传变异性。分别使用6个SRAP引物组合和4个AFLP引物组合，在SRAP中获得了总共183个扩增片段（等位基因）和1758个多态性片段（条带），在AFLP中获得了202个等位基因和716个条带。AFLP和SRAP标记的多态性信息含量（PIC）值高于0.8，表明在所测试的蚕豆基因型中存在相当数量的遗传多样性。基于UPGMA的蚕豆基因型聚类在很大程度上基于起源和/或遗传背景。基于SRAP的聚类分析结果显示相关性较弱且不显著，而基于AFLP分析与农艺形态性状的相关性则高度显著（分别为r = 0.01，p > 0.54和r = 0.26，p < 0.004）。联合使用SRAP和AFLP标记被证明在分子水平上对遗传多样性评估非常有用。它们表现出高鉴别力，能够高效且准确地区分蚕豆基因型。
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