Hao Qing, Liu Zheng-An, Shu Qing-Yan, Zhang Rui'e, De Rick Jan, Wang Liang-Sheng
Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Xiangshan, Haidian District, Beijing, PR China.
Hereditas. 2008 Feb;145(1):38-47. doi: 10.1111/j.0018-0661.2008.2013.x.
Plants of Paeonia are valuable for their ornamental and medicinal values. Genetic relations and hybrids identification among different sections of Paeonia were studied using sequence related amplified polymorphism (SRAP) markers. A total of 29 cultivars including 2 intersectional hybrids, 13 sect. Moutan and 14 from sect. Paeonia were used. A total of 197 bands were produced using 24 primer combinations, among which 187 bands showed polymorphism. From the bands amplified, we can identify the peony cultivars using unique SRAP markers and specific primer combinations. Fourteen peony cultivars were distinguished among each other by using totally 35 SRAP markers, which were generated by 16 primer pairs. Two specific primer pairs of Me8/Em8 and Me8/Em1 can be used to identify cultivars from different sections. The mean genetic similarity coefficient (GS), the gene diversity (GD), and the Shannon's information index of peony cultivars were 0.45, 0.19 and 0.32, respectively. Both UPGMA (unweighted pair-group method of arithmetic average) dendrogram and PCA (principle component analysis) analysis showed clear genetic relationships among the 29 peony cultivars, and within section and its intersectional hybrids. The above results are valuable for estimating and analyzing genetic background of Paeonia, parent selection in crossing breeding programs, molecular marker assisted selection (MAS) breeding for further germplasm innovation programs.
芍药属植物因其观赏价值和药用价值而备受重视。利用序列相关扩增多态性（SRAP）标记对芍药属不同组间的亲缘关系和杂种鉴定进行了研究。共使用了29个品种，包括2个组间杂种、13个牡丹组品种和14个芍药组品种。使用24对引物组合共产生了197条带，其中187条带表现出多态性。从扩增出的条带中，我们可以利用独特的SRAP标记和特定的引物组合来鉴定芍药品种。通过总共35个由16对引物产生的SRAP标记，区分了14个芍药品种。Me8/Em8和Me8/Em1这两对特异性引物可用于鉴定不同组的品种。芍药品种的平均遗传相似系数（GS）、基因多样性（GD）和香农信息指数分别为0.45、0.19和0.32。UPGMA（非加权算术平均法）聚类图和主成分分析（PCA）均显示了29个芍药品种以及组内和组间杂种之间清晰的遗传关系。上述结果对于评估和分析芍药属的遗传背景、杂交育种计划中的亲本选择以及进一步种质创新计划中的分子标记辅助选择（MAS）育种具有重要价值。
