Zhao Qingbo
Department of Emergency, 324 Hospital of The Chinese People's Liberation Army Chongqing 400020, China.
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1760-7. eCollection 2015.
Lung cancer ranks among one of the most frequent causes of cancer death in the world. Here, we investigated PRR11, one novel gene, with no functional annotation, was found to be over-expressed in lung cancer patients suggesting its potential implication in tumorigenesis. Furthermore, high PRR11 levels predict shorter survival of lung cancer patients. In this study, we investigated the therapeutic potential of PRR11 in lung cancer using the technique of RNA silencing via small interfering RNA (siRNA). Synthetic siRNA duplexes against PRR11 were introduced into 3 lung cancer cell lines, which subsequently resulted in a significant depletion in PRR11 expression in the cells. We found that the targeted depletion of PRR11 caused a dramatic cell cycle arrest followed by massive apoptotic cell death, and eventually resulted in a significant decrease in growth and viability of all 2 lung cancer cell lines. In summary, our study strongly suggests that PRR11 may serve as a potential therapeutic target in human lung cancer.
肺癌是全球癌症死亡的常见原因之一。在此，我们研究了PRR11，这是一个无功能注释的新基因，发现在肺癌患者中过度表达，提示其在肿瘤发生中可能发挥作用。此外，PRR11高水平预示肺癌患者生存期较短。在本研究中，我们通过小干扰RNA（siRNA）技术利用RNA沉默研究了PRR11在肺癌中的治疗潜力。针对PRR11的合成siRNA双链体被导入3种肺癌细胞系，随后导致细胞中PRR11表达显著降低。我们发现靶向敲低PRR11导致细胞周期显著停滞，随后大量细胞凋亡死亡，并最终导致所有2种肺癌细胞系的生长和活力显著下降。总之，我们的研究强烈提示PRR11可能是人类肺癌的潜在治疗靶点。
