Sarioglu Nurhan, Bilen Cigdem, Sackes Zubeyde, Gencer Nahit
Department of Pulmonary Diseases, Faculty of Medicine, Balikesir University Balikesir and.
Arch Physiol Biochem. 2015 May;121(2):56-61. doi: 10.3109/13813455.2015.1011068. Epub 2015 May 14.
Carbonic anhydrase (CA) is an enzyme which plays role/roles in various homeostatic mechanisms, such as the acid-base balance and electrolyte secretion in various tissues. This study aimed to determine and to compare possible alterations in activity of this enzyme caused by use of bronchodilator drugs and respiratory infection antibiotics. CA I and II were purified from human erythrocytes by a simple one step procedure using Sepharose 4B-L-tyrosine-sulfonamide affinity column. The iso-enzymes were purified 259.16-fold with a yield of 31.74%. CAI and II isozymes were treated with several drugs, then the inhibition or activation of the enzymes were determined. The results of this study show that itrapropium bromide is the most effective inhibitor for human erythrocytes carbonic anhydrase compared with the other bronchodilator drugs.
碳酸酐酶（CA）是一种在多种稳态机制中发挥作用的酶，如各种组织中的酸碱平衡和电解质分泌。本研究旨在确定并比较使用支气管扩张剂药物和呼吸道感染抗生素引起的该酶活性的可能变化。通过使用琼脂糖4B-L-酪氨酸-磺酰胺亲和柱的简单一步法从人红细胞中纯化出CA I和II。同工酶纯化了259.16倍，产率为31.74%。用几种药物处理CAI和II同工酶，然后测定酶的抑制或激活情况。本研究结果表明，与其他支气管扩张剂药物相比，异丙托溴铵是对人红细胞碳酸酐酶最有效的抑制剂。
