McGarvey J A, Han R, Connell J H, Stanker L H, Hnasko R
Foodborne Toxin Detection and Protection Research Unit, Agricultural Research Service, United States Department of Agriculture, Albany, CA, USA.
Department of Plant Sciences, University of California, Davis, CA, USA.
J Appl Microbiol. 2015 Aug;119(2):529-38. doi: 10.1111/jam.12850. Epub 2015 Jun 16.
To compare the bacterial populations on organically and conventionally grown almond drupes before and after hull split.
We constructed 16S rRNA gene libraries, containing approx. 3000 sequences each, from the bacteria from organically and conventionally grown drupes before and after hull split. We observed that before hull split both conventionally and organically grown drupes were colonized by relatively few types of bacteria that were mostly common phyllosphere-associated Proteobacteria. However, the organically grown drupes contained significantly more Alphaproteobacteria and the conventionally grown drupes contained significantly more Gammaproteobacteria. The conventionally grown drupes also contained significantly more sequences associated with the phylum Actinobacteria. After hull split, we observed a significant increase in bacterial diversity, with many newly appearing sequences that were not normally associated with the phyllosphere.
Organic and conventional growing methodologies influence the types of bacteria on almond drupes and hull split results in a burst of microbial diversification.
Production of organic produce is increasing due to consumer preferences, but it was unknown how this methodology affects the bacterial populations on almond drupes. This is the first study to compare the bacterial populations of organically and conventionally grown almond drupes.
比较有机种植和传统种植的杏仁核果在果壳裂开前后的细菌群落。
我们构建了16S rRNA基因文库，每个文库包含约3000个序列，这些序列来自有机种植和传统种植的核果在果壳裂开前后的细菌。我们观察到，在果壳裂开前，传统种植和有机种植的核果都被相对较少种类的细菌定殖，这些细菌大多是常见的与叶际相关的变形菌。然而，有机种植的核果含有显著更多的α-变形菌，而传统种植的核果含有显著更多的γ-变形菌。传统种植的核果还含有显著更多与放线菌门相关的序列。果壳裂开后，我们观察到细菌多样性显著增加，出现了许多通常与叶际无关的新序列。
有机种植和传统种植方法会影响杏仁核果上的细菌种类，果壳裂开导致微生物多样性激增。
由于消费者的偏好，有机农产品的产量正在增加，但这种方法如何影响杏仁核果上的细菌群落尚不清楚。这是第一项比较有机种植和传统种植的杏仁核果细菌群落的研究。
