Wyatt Timon T, Gerwig Gerrit J, Kamerling Johannis P, Wösten Han A B, Dijksterhuis Jan
CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Carbohydr Res. 2015 Jun 26;411:49-55. doi: 10.1016/j.carres.2015.04.006. Epub 2015 Apr 20.
Different fungi, including the genera Neosartorya, Byssochlamys and Talaromyces, produce (asco)spores that survive pasteurization treatments and are regarded as the most stress-resistant eukaryotic cells. Here, the NMR analysis of a series of trehalose-based oligosaccharides, being compatible solutes that are accumulated to high levels in ascospores of the fungus Neosartorya fischeri, is presented. These oligosaccharides consist of an α,α-trehalose backbone, extended with one [α-D-Glcp-(1 → 6)-α-D-Glcp-(1 ↔ 1)-α-D-Glcp; isobemisiose], two [α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → 6)-α-D-Glcp-(1 ↔ 1)-α-D-Glcp] or three [α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → 6)-α-D-Glcp-(1 ↔ 1)-α-D-Glcp] glucose units. The tetra- and pentasaccharide, dubbed neosartose and fischerose, respectively, have not been reported before to occur in nature.
包括新萨托菌属、丝衣霉属和拟青霉属在内的不同真菌会产生(子囊)孢子,这些孢子能在巴氏杀菌处理后存活,被视为最具抗逆性的真核细胞。在此,我们展示了对一系列基于海藻糖的寡糖的核磁共振分析,这些寡糖是相容性溶质,在费氏新萨托菌的子囊孢子中大量积累。这些寡糖由一个α,α-海藻糖主链组成,并分别连接一个[α-D-葡萄糖-(1→6)-α-D-葡萄糖-(1↔1)-α-D-葡萄糖;异蜜二糖]、两个[α-D-葡萄糖-(1→6)-α-D-葡萄糖-(1→6)-α-D-葡萄糖-(1↔1)-α-D-葡萄糖]或三个[α-D-葡萄糖-(1→6)-α-D-葡萄糖-(1→6)-α-D-葡萄糖-(1→6)-α-D-葡萄糖-(1↔1)-α-D-葡萄糖]葡萄糖单元。四糖和五糖分别被称为新萨托糖和费氏糖,此前尚未有在自然界中存在的报道。