Tang Mei-Qiong, Min Dan-Dan, Li Gang, Jiang Ni, Ye Yun-Feng
Yao Xue Xue Bao. 2015 Feb;50(2):227-32.
With homology cloning approaches coupling with RACE (rapid-amplification of cDNA ends) techniques, the full-length coding sequence of pathogenesis-related protein PR10-1 with differential expression was cloned from the total RNA of the root of Panax notoginseng, and its function was explored furtherly. As a result, the longest 465 bp ORF (named as PnPR10-1 with the Accession No. KJ741402 in GenBank) was detected from the cloned sequence with full-length of cDNA of 863 bp. The corresponding peptide encoded consisted of 155 amino acids, contained some domains such as Bet-v-I, and showed high similarity with that from Panax ginseng by analysis of phylogenetic trees created from the alignments. Real-time quantitative PCR showed that the expression of PnPR10-1 gene was constitutive in different tissues of 1-3 year old plant, suggesting that it might be involved in growth, development, and secondary metabolism; yet it was up-regulated significantly with the infection of Fusarium oxysporum in root, suggesting that it might be involved in defense against many diseases including root rot in P. notoginseng.
采用同源克隆方法结合RACE（cDNA末端快速扩增）技术，从不同表达的三七根总RNA中克隆了病程相关蛋白PR10-1的全长编码序列，并对其功能进行了进一步探究。结果，从克隆的863 bp全长cDNA序列中检测到最长465 bp的开放阅读框（命名为PnPR10-1，在GenBank中的登录号为KJ741402）。编码的相应肽由155个氨基酸组成，包含一些如Bet-v-I等结构域，通过比对构建的系统发育树分析显示与人参的该蛋白具有高度相似性。实时定量PCR表明，PnPR10-1基因在1-3年生植株的不同组织中组成性表达，表明它可能参与生长、发育和次生代谢；然而，它在根部受尖孢镰刀菌感染时显著上调，表明它可能参与三七对包括根腐病在内的多种病害的防御。
