Lee Young-Sil, Choi Bong-Keun, Lee Hae Jin, Lee Dong-Ryung, Cheng Jinhua, Lee Won-Keun, Yang Seung Hwan, Suh Joo-Won
Center for Nutraceutical and Pharmaceutical Materials, Myongji University, 116 Myongji-Ro, Cheoin-Gu, Yongin-Si, Gyeonggi-Do 449-728, Korea.
Interdisciplinary Program of Biomodulation, Myongji University, 116 Myongji-Ro, Cheoin-Gu, Yongin-Si, Gyeonggi-Do 449-728, Korea.
Asian Pac J Trop Med. 2015 Apr;8(4):276-82. doi: 10.1016/S1995-7645(14)60330-8.
To explore the anti-obesity effects and the mechanism of action of Monascus pilosus(M. pilosus)-fermented black soybean (MFBS) extracts (MFBSE) and MFBS powders (MFBSP) in adipocytes and high-fat diet (HFD)-induced obese mice, respectively.
Black soybean was fermented with M. pilosus, and the main constituents in MFBS were analyzed by HPLC analysis. In vitro, MFBSE were examined for anti-adipogenic effects using Oil-Red O staining. In vivo, mice were fed a normal-fat diet (NFD) control, HFD control or HFD containing 1 g/kg MFBSP for 12 weeks, and then body weight gain and tissues weight measured. Real-time PCR and western blot assay were used to determine the mechanism of anti-adipogenic effects.
MFBSE inhibited lipid accumulation in 3T3-L1 adipocytes without exerting cell cytotoxicity. MFBSP treatment in HFD-fed mice significantly decreased the body weight gain compared with the HFD control mice. MFBSE and MFBSP treatment resulted in significantly lower mRNA levels of adipogenesis-related genes, such as peroxisome proliferator-activated receptor γ(PPAR γ), fatty acid-binding protein 4 (FABP4), and fatty acid synthase (FAS), in adipocytes and in white adipose tissue (WAT) of HFD-induced obese mice.
These results suggest that the anti-obesity effects of MFBS are elicited by regulating the expression of adipogenesis-related genes in adipocytes and WAT of HFD-induced obese mice.
分别探讨毛霉发酵黑豆提取物（MFBSE）和毛霉发酵黑豆粉（MFBSP）在脂肪细胞和高脂饮食（HFD）诱导的肥胖小鼠中的抗肥胖作用及其作用机制。
用毛霉发酵黑豆，采用高效液相色谱分析法分析MFBSP中的主要成分。体外实验，采用油红O染色检测MFBSE的抗脂肪生成作用。体内实验，将小鼠分为正常脂肪饮食（NFD）对照组、HFD对照组或含1 g/kg MFBSP的HFD组，喂养12周，然后测量体重增加量和组织重量。采用实时荧光定量聚合酶链反应（Real-time PCR）和蛋白质免疫印迹法（western blot）检测抗脂肪生成作用的机制。
MFBSE可抑制3T3-L1脂肪细胞中的脂质积累，且不产生细胞毒性。与HFD对照小鼠相比，MFBSP处理的HFD喂养小鼠体重增加明显减少。MFBSE和MFBSP处理可使HFD诱导的肥胖小鼠脂肪细胞和白色脂肪组织（WAT）中脂肪生成相关基因的mRNA水平显著降低，如过氧化物酶体增殖物激活受体γ（PPARγ）、脂肪酸结合蛋白4（FABP4）和脂肪酸合酶（FAS）。
这些结果表明，MFBSP的抗肥胖作用是通过调节HFD诱导的肥胖小鼠脂肪细胞和WAT中脂肪生成相关基因的表达来实现的。
