Vav3对人胃癌SGC7901细胞增殖的影响及机制
[Effect and mechanism of Vav3 on the proliferation of human gastric cancer SGC7901 cells].
作者信息
Tan Bibo, Li Yong, Fan Liqiao, Zhao Qun, Wang Dong, Liu Yu, Li Zhaoxing
机构信息
the Third Department of Surgery, the Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China.
the Third Department of Surgery, the Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China; Email:
出版信息
Zhonghua Zhong Liu Za Zhi. 2015 Mar;37(3):175-80.
OBJECTIVE
The purpose of this study was to investigate the effect and mechanism of Vav3 gene on the proliferation of human gastric cancer cell line SGC7901.
METHODS
The expressions of Vav3 proten in gastric cancer tissue, tumor-adjacent tissue, human gastric cancer cell line SGC7901 and gastric epithelial cell line GES-1 cells were tested by Western blot. Vav3-siRNA was transfected into the SGC7901 cells. The proliferation of SGC7901 cells in vitro was measured by MTT assay. Cell cycle of SGC7901 cells was determined by flow cytometry.The expressions of proliferation-related genes PCNA, p16, cyclin D1, Rb were determined by qPCR and Western blot assay. Orthotopic transplantation nude mouse models of gastric cancer were prepared, and the tumor growth and expressions of PCNA, P16, cyclin D1, and Rb proteins were examined.
RESULTS
The relative expressions of Vav3 in the gastric cancer and peritumoral tissue were 0.910±0.242 and 0.243±0.045, respectively; the relative expressions of Vav3 in SGC7901 and GSE-1 cells were 0.925±0.127 and 0.277±0.038, respevtively (both P<0.05). The expression of Vav3 protein in SGC7901 cells was effectively inhibited by Vav3-siRNA. Proliferation of SGC7901 cells was inhibited by (83.43±10.17)% after 80 nmol/L Vav3-siRNA transfection (P<0.05). The ratio of SGC7901 cells in G0/G1 phase was increased, and in S phase decreased after Vav3-siRNA transfection (both P<0.05). The expressions of PCNA and cyclin D1 were decreased in cells after Vav3-siRNA transfection, and expressions of p16 and Rb were increased after Vav3-siRNA transfection (P<0.05 for all). The tumor growth in the Vav3-siRNA group was much slower than that in the other 2 control groups of nude mouse models. Compared with the two control groups, expressions of PCNA and cyclin D1 were significantly lower in the Vav3-siRNA group, while expressions of p16 and Rb were increased (P<0.05 for all).
CONCLUSION
Vav3 can promote the proliferation of gastric cancer cells by regulating proliferation-related genes.
目的
本研究旨在探讨Vav3基因对人胃癌细胞系SGC7901增殖的影响及其机制。
方法
采用蛋白质免疫印迹法检测Vav3蛋白在胃癌组织、癌旁组织、人胃癌细胞系SGC7901和胃上皮细胞系GES-1细胞中的表达。将Vav3-siRNA转染至SGC7901细胞。采用MTT法检测SGC7901细胞的体外增殖情况。通过流式细胞术检测SGC7901细胞的细胞周期。采用实时荧光定量PCR和蛋白质免疫印迹法检测增殖相关基因PCNA、p16、细胞周期蛋白D1、Rb的表达。制备胃癌原位移植裸鼠模型，观察肿瘤生长情况，并检测PCNA、P16、细胞周期蛋白D1和Rb蛋白的表达。
结果
Vav3在胃癌组织和癌旁组织中的相对表达量分别为0.910±0.242和0.243±0.045；在SGC7901和GSE-1细胞中的相对表达量分别为0.925±0.127和0.277±
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