Pirson Marc, Knoops Bernard
Group of Animal Molecular and Cellular Biology, Institut des Sciences de la Vie (ISV), Université catholique de Louvain, 1348, Louvain-la-Neuve, Belgium.
J Comp Neurol. 2015 Dec 1;523(17):2599-617. doi: 10.1002/cne.23807. Epub 2015 Jun 8.
Reactive oxygen and nitrogen species (ROS/RNS) are natural byproducts of cellular metabolism. Although these molecules are deleterious at high concentrations, moderate levels of ROS/RNS are essential for normal cell function and take part in numerous cellular processes. The regulation of ROS/RNS is largely attended by peroxiredoxins (Prdxs) and their main reductants, thioredoxins (Trxs). Through their oxidoreductase activities, the members of the Trx/Prdx system can also affect certain cellular processes, notably many implicated in central nervous system (CNS) development. Although several studies have investigated the expression of Prdxs and Trxs in mouse, rat, and human adult CNS, few data are available concerning embryonic stages. In this work, we use immunofluorescence analyses to study the distribution of these enzymes during prenatal mouse spinal cord development. Our results highlight several patterns that contrast with available data for the adult. Indeed, Prdx1, Prdx4, and Prdx6, which are expressed in glial cells in the adult CNS, present clear neuronal localization in mouse spinal cord during embryonic development. Additionally, Prdx1, Prdx2, and to a lesser extent Prdx4, Prdx6, and Trx1 are localized mainly in the nucleus of neural cells. Finally, we identified a consistent, intense expression of all Prdxs and Trxs in groups of cells located in ventral regions of the spinal cord that express motor neuronal markers. These striking expression patterns suggest novel functions of these enzymes at these stages and offer clues to the role of the Trx/Prdx system during embryonic development of the spinal cord.
活性氧和氮物种（ROS/RNS）是细胞代谢的天然副产物。尽管这些分子在高浓度时具有有害性，但适度水平的ROS/RNS对于正常细胞功能至关重要，并参与众多细胞过程。ROS/RNS的调节在很大程度上由过氧化物还原酶（Prdxs）及其主要还原剂硫氧还蛋白（Trxs）负责。通过其氧化还原酶活性，Trx/Prdx系统的成员也可以影响某些细胞过程，特别是许多与中枢神经系统（CNS）发育有关的过程。尽管有几项研究调查了Prdxs和Trxs在小鼠、大鼠和人类成年CNS中的表达，但关于胚胎阶段的数据很少。在这项工作中，我们使用免疫荧光分析来研究这些酶在小鼠产前脊髓发育过程中的分布。我们的结果突出了几种与成年期现有数据形成对比的模式。事实上，在成年CNS的神经胶质细胞中表达的Prdx1、Prdx4和Prdx6在胚胎发育期间的小鼠脊髓中呈现出明显的神经元定位。此外，Prdx1、Prdx2以及在较小程度上的Prdx4、Prdx6和Trx1主要定位于神经细胞的细胞核中。最后，我们在位于脊髓腹侧区域、表达运动神经元标志物的细胞群中鉴定出所有Prdxs和Trxs一致且强烈的表达。这些显著的表达模式表明这些酶在这些阶段具有新功能，并为Trx/Prdx系统在脊髓胚胎发育过程中的作用提供了线索。
