∥Dongsuh Foods Corporation, 203 Bupyeongbuk-ro, Bupyeong-gu, Incheon 403-858, Korea.
⊥Department of Food and Biotechnology, Hoseo University, 79-20 Hoseoro, Asan, Chungnam 336-795, Korea.
J Agric Food Chem. 2015 Jun 3;63(21):5266-72. doi: 10.1021/acs.jafc.5b01495. Epub 2015 May 21.
To enhance the oral bioaccessibility of flavonoids, including quercetin, naringenin, and hesperetin, we prepared an edible oil-based lipid nanoparticle (LNP) system. Flavonoid-loaded LNPs were similar to the blank LNP in physicochemical characteristics (z average <154.8 nm, polydispersity index <0.17, and ζ potential < -40.8 mV), and their entrapment efficiency was >81% at 0.3 wt % flavonoid concentration of the lipid phase. In the simulated digestion assay (mouth, stomach, and small intestine), LNPs were hydrolyzed under small intestine conditions and protected successfully incorporated flavonoids (≥94%). Moreover, the relative bioaccessibility of flavonoids was >71%, which was otherwise <15%, although flavonoids were released rapidly from LNPs into the medium. In conclusion, since the flavonoids incorporated in LNPs were preserved well during oral digestion and had improved bioaccessibility, the designed LNP system may serve as an encapsulation strategy to enhance the bioavailability of nonbioaccessible nutraceuticals in foods.
为了提高包括槲皮素、柚皮素和橙皮素在内的类黄酮的口服生物利用度，我们制备了一种基于食用油的脂质纳米粒（LNP）系统。负载类黄酮的 LNPs 在理化性质上与空白 LNP 相似（Z 均数<154.8nm，多分散指数<0.17，ζ 电位<-40.8mV），并且在脂质相的类黄酮浓度为 0.3wt%时，包封效率>81%。在模拟消化试验（口腔、胃和小肠）中，LNPs 在小肠条件下被水解，并成功保护了所包含的类黄酮（≥94%）。此外，类黄酮的相对生物利用度>71%，而未经包封的类黄酮的生物利用度<15%，尽管 LNPs 中的类黄酮很快从 LNPs 释放到介质中。总之，由于在口服消化过程中 LNP 中包含的类黄酮得到了很好的保护并且生物利用度得到了提高，因此所设计的 LNP 系统可以作为一种包封策略，以提高食品中非生物可利用的营养保健品的生物利用度。
