Rahul C N, Shiva Krishna K, Meera M, Phadke Sandhya, Rajesh Vidya
Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Hyderabad Campus, Andhra Pradesh, India.
Sir Ronald Ross Institute of Tropical and Communicable Diseases, Hyderabad, Andhra Pradesh, India.
Blood Cells Mol Dis. 2015 Jun;55(1):30-5. doi: 10.1016/j.bcmd.2015.03.013. Epub 2015 Mar 31.
Worldwide malaria risk due to Plasmodium vivax makes development of vaccine against P. vivax, a high priority. Serine Repeat Antigen of P. vivax (PvSERA) is a multigene family of blood stage proteins with 12 homologues. Sequence diversity studies are important for understanding them as potential vaccine candidates. No information on N-terminal diversity of these genes is available in literature. In this paper, we evaluate the genetic polymorphism of N-terminal regions of the highly expressed member PvSERA4 and PvSERA5 genes from Indian field isolates. Our results show that PvSERA4 has deletions and insertions in Glutamine rich tetrameric repeat units contributing to its diversity. PvSERA5 also exhibits high genetic diversity with non-synonymous substitutions leading to identification of novel haplotypes from India. Our first report helps in elucidating the allelic variants of PvSERA genes in this region and contributes to evaluating their efficacy as vaccine candidates.
由于间日疟原虫导致的全球疟疾风险使得开发针对间日疟原虫的疫苗成为高度优先事项。间日疟原虫丝氨酸重复抗原（PvSERA）是一个血期蛋白的多基因家族，有12个同源物。序列多样性研究对于将它们理解为潜在的疫苗候选物很重要。文献中没有关于这些基因N端多样性的信息。在本文中，我们评估了来自印度野外分离株的高表达成员PvSERA4和PvSERA5基因N端区域的遗传多态性。我们的结果表明，PvSERA4在富含谷氨酰胺的四聚体重复单元中有缺失和插入，这促成了其多样性。PvSERA5也表现出高遗传多样性，非同义替换导致从印度鉴定出新的单倍型。我们的首次报告有助于阐明该区域PvSERA基因的等位基因变体，并有助于评估它们作为疫苗候选物的效力。
