Trif Mihaela, Florian Paula E, Roseanu Anca, Moisei Magdalena, Craciunescu Oana, Astete Carlos E, Sabliov Cristina M
Institute of Biochemistry of the Romanian Academy, Bucharest, 060031, Romania.
Department of Cellular Biology, National Institute R&D for Biological Sciences, Bucharest, 060031, Romania.
J Biomed Mater Res A. 2015 Nov;103(11):3599-611. doi: 10.1002/jbm.a.35498. Epub 2015 Jun 11.
Polymeric nanoparticles (NPs) are known to facilitate intracellular uptake of drugs to improve their efficacy, with minimum bioreactivity. The goal of this study was to assess cellular uptake and trafficking of PLGA NPs and chitosan (Chi)-covered PLGA NPs in Madin-Darby bovine kidney (MDBK) and human colorectal adenocarcinoma (Colo 205) cells. Both PLGA and Chi-PLGA NPs were not cytotoxic to the studied cells at concentrations up to 2500 μg/mL. The positive charge conferred by the chitosan deposition on the PLGA NPs improved NPs uptake by MDBK cells. In this cell line, Chi-PLGA NPs colocalized partially with early endosomes compartment and showed a more consistent perinuclear localization than PLGA NPs. Kinetic uptake of PLGA NPs by Colo 205 was slower than that by MDBK cells, detected only at 24 h, exceeding that of Chi-PLGA NPs. This study offers new insights on NP interaction with target cells supporting the use of NPs as novel nutraceuticals/drug delivery systems in metabolic disorders or cancer therapy. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3599-3611, 2015.
已知聚合物纳米颗粒（NPs）可促进药物的细胞内摄取，以提高其疗效，同时生物反应性最小。本研究的目的是评估聚乳酸-羟基乙酸共聚物纳米颗粒（PLGA NPs）和壳聚糖（Chi）包被的PLGA NPs在马-达二氏牛肾（MDBK）细胞和人结肠腺癌（Colo 205）细胞中的细胞摄取和转运情况。在浓度高达2500μg/mL时，PLGA NPs和Chi-PLGA NPs对所研究的细胞均无细胞毒性。壳聚糖沉积在PLGA NPs上赋予的正电荷提高了MDBK细胞对NPs的摄取。在该细胞系中，Chi-PLGA NPs与早期内体区室部分共定位，并且与PLGA NPs相比显示出更一致的核周定位。Colo 205细胞对PLGA NPs的动力学摄取比MDBK细胞慢，仅在24小时时检测到，且超过了Chi-PLGA NPs的摄取。本研究为NP与靶细胞的相互作用提供了新的见解，支持将NPs用作代谢紊乱或癌症治疗中的新型营养保健品/药物递送系统。©2015威利期刊公司。《生物医学材料研究杂志》A部分：103A：3599 - 3611，2015年。
