Zhao Panfeng, Zhang Lida, Zhao Lingxia
Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
BMC Plant Biol. 2015 May 15;15:119. doi: 10.1186/s12870-015-0492-7.
Tomato (Solanum lycopersicum) self-compatibility (SC) is defined as self-pollen tubes that can penetrate their own stigma, elongate in the style and fertilize their own ovules. Self-incompatibility (SI) is defined as self-pollen tubes that are prevented from developing in the style. To determine the influence of gene expression on style self-pollination, a transcriptome-wide comparative analysis of SC and SI tomato unpollinated/pollinated styles was performed using RNA-sequencing (RNA-seq) data.
Transcriptome profiles of 24-h unpollination (UP) and self-pollination (P) styles from SC and SI tomato species were generated using high-throughput next generation sequencing. From the comparison of SC self-pollinated and unpollinated styles, 1341 differentially expressed genes (DEGs) were identified, of which 753 were downregulated and 588 were upregulated. From the comparison of SI self-pollinated and unpollinated styles, 804 DEGs were identified, of which 215 were downregulated and 589 were upregulated. Nine gene ontology (GO) terms were enriched significantly in SC and 78 GO terms were enriched significantly in SI. A total of 105 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were identified in SC and 80 enriched KEGG pathways were identified in SI, among which "Cysteine and methionine metabolism pathway" and "Plant hormone signal transduction pathway" were significantly enriched in SI.
This study is the first global transcriptome-wide comparative analysis of SC and SI tomato unpollinated/pollinated styles. Advanced bioinformatic analysis of DEGs uncovered the pathways of "Cysteine and methionine metabolism" and "Plant hormone signal transduction", which are likely to play important roles in the control of pollen tubes growth in SI species.
番茄（Solanum lycopersicum）的自交亲和性（SC）被定义为自花花粉管能够穿透自身柱头，在花柱中伸长并使自身胚珠受精。自交不亲和性（SI）被定义为自花花粉管在花柱中发育受阻。为了确定基因表达对花柱自花授粉的影响，利用RNA测序（RNA-seq）数据对自交亲和与自交不亲和番茄未授粉/授粉花柱进行了全转录组比较分析。
利用高通量下一代测序技术生成了自交亲和与自交不亲和番茄品种24小时未授粉（UP）和自花授粉（P）花柱的转录组图谱。通过比较自交亲和番茄自花授粉和未授粉花柱，鉴定出1341个差异表达基因（DEG），其中753个下调，588个上调。通过比较自交不亲和番茄自花授粉和未授粉花柱，鉴定出804个DEG，其中215个下调，589个上调。9个基因本体（GO）术语在自交亲和番茄中显著富集，78个GO术语在自交不亲和番茄中显著富集。在自交亲和番茄中总共鉴定出105条富集的京都基因与基因组百科全书（KEGG）通路，在自交不亲和番茄中鉴定出80条富集的KEGG通路，其中“半胱氨酸和甲硫氨酸代谢途径”和“植物激素信号转导途径”在自交不亲和番茄中显著富集。
本研究首次对自交亲和与自交不亲和番茄未授粉/授粉花柱进行了全转录组比较分析。对差异表达基因的高级生物信息学分析揭示了“半胱氨酸和甲硫氨酸代谢”和“植物激素信号转导”途径，它们可能在自交不亲和物种中花粉管生长的控制中发挥重要作用。
