采用核壳颗粒柱快速测定饮料中的生物胺。
Fast determination of biogenic amines in beverages by a core-shell particle column.
作者信息
Preti Raffaella, Antonelli Marta Letizia, Bernacchia Roberta, Vinci Giuliana
机构信息
Laboratory of Commodity Science, Department of Management, Sapienza University of Rome, via del Castro Laurenziano 9, 00161 Rome, Italy.
Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00161 Rome, Italy.
出版信息
Food Chem. 2015 Nov 15;187:555-62. doi: 10.1016/j.foodchem.2015.04.075. Epub 2015 Apr 24.
A fast and reliable HPLC method for the determination of 11 biogenic amines in beverages has been performed. After pre-column derivatization with dansyl-chloride a Kinetex C18 core-shell particle column (100 mm × 4.6 mm, 2.6 μm particle size) has been employed and the biogenic amines were identified and quantified in a total run time of 13 min with ultraviolet (UV) or fluorescence detection (FLD). Chromatographic conditions such as column temperature (kept at 50 °C), gradient elution and flow rate have been optimized and the method has been tested on red wine and fruit nectar. The proposed method is enhanced in terms of reduced analysis time and eluent consumption with respect of classical HPLC method as to be comparable to UHPLC methods. Green and cost-effective, this method can be used as a quality-control tool for routine quantitative analysis of biogenic amines in beverages for the average laboratory.
已建立一种快速可靠的HPLC方法用于测定饮料中的11种生物胺。用丹磺酰氯进行柱前衍生后，使用了Kinetex C18核壳颗粒柱（100 mm×4.6 mm，粒径2.6μm），并在13分钟的总运行时间内通过紫外（UV）或荧光检测（FLD）对生物胺进行了鉴定和定量。对柱温（保持在50℃）、梯度洗脱和流速等色谱条件进行了优化，并在红酒和水果花蜜上对该方法进行了测试。与经典HPLC方法相比，该方法在分析时间和洗脱液消耗方面有所改进，可与超高效液相色谱（UHPLC）方法相媲美。该方法绿色且经济高效，可作为普通实验室对饮料中生物胺进行常规定量分析的质量控制工具。
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