Wei HaiYan, Yang Fei, Wang Yu, Zhou YuanLong, Yan Yan, Liang GeYu, Yin LiHong, Pu YuePu
a Key Laboratory of Environmental Medicine Engineering of Ministry of Education , School of Public Health, Southeast University , Nanjing 210009 , People's Republic of China.
b Department of Occupational and Environmental Health , School of Public Health, Central South University , Changsha , Hunan 410078 , People's Republic of China.
Environ Technol. 2015;36(21):2796-802. doi: 10.1080/09593330.2015.1049213. Epub 2015 Jun 4.
Electrospun polymer nanofibres were used as novel solid-phase extraction (SPE) sorbents to extract and quantify the microcystins (MCs) including microcystin-RR (MC-RR) and microcystin-LR (MC-LR) from in-suit water samples. The parameters that influenced the extraction efficiency were studied, including the amount of nanofibre, eluted solvent, eluted volume, pH, and the water sample volume. Under optimized conditions, a linear response for MC-RR and MC-LR over the range of 0.25-4 µg/L was achieved with r(2) values of 0.998 and 0.997, respectively. The extraction recovery of MC-RR and MC-LR was 97-102% and 98-100%, respectively, when the MC concentration was 0.25-4 µg/L. When their concentrations ranged from 0.05 to 0.25 µg/L, the MCs could be detected with high accuracy by the nanofibre SPE sorbent combined with nitrogen gas. Due to its simplicity, environment-friendliness, high efficiency, reusability, and sensitivity, the electrospun polymer nanofibre can be applied as a novel SPE sorbent to extract and detect the MCs from in-suit water samples.
静电纺丝聚合物纳米纤维被用作新型固相萃取（SPE）吸附剂，用于从原位水样中萃取和定量微囊藻毒素（MCs），包括微囊藻毒素-RR（MC-RR）和微囊藻毒素-LR（MC-LR）。研究了影响萃取效率的参数，包括纳米纤维的用量、洗脱溶剂、洗脱体积、pH值和水样体积。在优化条件下，MC-RR和MC-LR在0.25-4μg/L范围内呈线性响应，r(2)值分别为0.998和0.997。当MC浓度为0.25-4μg/L时，MC-RR和MC-LR的萃取回收率分别为97-102%和98-100%。当它们的浓度范围为0.05-0.25μg/L时，纳米纤维SPE吸附剂结合氮气可高精度检测MCs。由于其操作简单、环境友好、高效、可重复使用和灵敏度高，静电纺丝聚合物纳米纤维可作为一种新型SPE吸附剂，用于从原位水样中萃取和检测MCs。
