影响生物银行中人类生物样本完整性的分析前变量。
Preanalytical variables affecting the integrity of human biospecimens in biobanking.
机构信息
Department of Research, Nykoebing Falster Hospital, Nykoebing Falster, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Department of Laboratory Medicine, Boston Children's Hospital, Boston, MA;
International Society for Biological and Environmental Repositories, Vancouver, Canada; Editor-in-Chief, Biopreservation and Biobanking, Vancouver, Canada.
出版信息
Clin Chem. 2015 Jul;61(7):914-34. doi: 10.1373/clinchem.2014.228783. Epub 2015 May 15.
BACKGROUND
Most errors in a clinical chemistry laboratory are due to preanalytical errors. Preanalytical variability of biospecimens can have significant effects on downstream analyses, and controlling such variables is therefore fundamental for the future use of biospecimens in personalized medicine for diagnostic or prognostic purposes.
CONTENT
The focus of this review is to examine the preanalytical variables that affect human biospecimen integrity in biobanking, with a special focus on blood, saliva, and urine. Cost efficiency is discussed in relation to these issues.
SUMMARY
The quality of a study will depend on the integrity of the biospecimens. Preanalytical preparations should be planned with consideration of the effect on downstream analyses. Currently such preanalytical variables are not routinely documented in the biospecimen research literature. Future studies using biobanked biospecimens should describe in detail the preanalytical handling of biospecimens and analyze and interpret the results with regard to the effects of these variables.
背景
临床化学实验室中的大多数错误都是由于分析前误差导致的。生物样本的分析前变异性会对下游分析产生重大影响，因此控制这些变量对于未来将生物样本用于个性化医疗的诊断或预后目的至关重要。
内容
本综述的重点是检查影响生物库中人类生物样本完整性的分析前变量，特别关注血液、唾液和尿液。还讨论了与这些问题相关的成本效益。
总结
研究的质量将取决于生物样本的完整性。应考虑对下游分析的影响来规划分析前准备。目前，生物样本研究文献中通常没有记录这些分析前变量。未来使用生物样本库的研究应详细描述生物样本的分析前处理，并根据这些变量的影响来分析和解释结果。
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