Sato M, Yoshizaki K, Koyano H
Department of Physiology, Akita University School of Medicine, Japan.
Brain Res. 1989 Dec 11;504(1):132-5. doi: 10.1016/0006-8993(89)91609-0.
It was examined whether or not 22Na+ influx into cultured cells of the carotid body (CB) from newborn rabbits might be stimulated by veratridine (VRT), using superior cervical ganglion (SCG) cells as a standard, showing the VRT-stimulating effects on 22Na+ influx. In a CB glomus cell-rich culture, VRT induced a 22Na+ influx increase, as seen in a SCG neuronal cell-rich culture, which was entirely inhibited by tetrodotoxin (TTX). In contrast, in a CB non-glomus cell culture as well as in a SCG non-neuronal cell culture, the VRT-stimulating effect was not seen. This indicates that the VRT-stimulating effect found in the CB glomus cell-rich culture was evoked from only glomus cells. It is concluded that glomus cells have TTX-sensitive voltage-dependent sodium channels, which might be indirectly involved in the chemotransduction mechanism in the CB.
以颈上神经节（SCG）细胞作为标准，研究了来自新生兔的颈动脉体（CB）培养细胞中的22Na+内流是否会受到藜芦碱（VRT）的刺激，SCG细胞显示出VRT对22Na+内流的刺激作用。在富含CB球细胞的培养物中，VRT引起22Na+内流增加，这与富含SCG神经元细胞的培养物中观察到的情况相同，且这种增加完全被河豚毒素（TTX）抑制。相反，在CB非球细胞培养物以及SCG非神经元细胞培养物中，未观察到VRT刺激作用。这表明在富含CB球细胞的培养物中发现的VRT刺激作用仅由球细胞引起。得出的结论是，球细胞具有TTX敏感的电压依赖性钠通道，这可能间接参与了CB中的化学转导机制。
