Ren Shukun, Sugimoto Yuki, Kobayashi Taichi, Masuda Shinji
Center for Biological Resources & Informatics, Tokyo Institute of Technology, Yokohama, Japan.
Graduate School of Bioscience & Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
FEBS Lett. 2015 Jul 8;589(15):1879-82. doi: 10.1016/j.febslet.2015.05.019. Epub 2015 May 14.
PixD is a blue light using flavin (BLUF)-type blue-light photoreceptor controlling phototaxis in the cyanobacterium Synechocystis sp. PCC6803. The crystal structure of PixD shows a decamer, although in solution an equilibrium is maintained between the dimer and decamer. Because the ratio of these two conformers is altered by illumination, the equilibrium state determines photosensitivity. However, no structural information is available for the PixD dimer. Here, we report a predicted structure for the dimer based on docking simulation, mutagenesis, and mass spectrometry-based cross-linking analyses. The results indicate the importance of the PixD C-terminus for dimer preference and photosensitivity.
PixD是一种利用黄素（BLUF）型蓝光感受器的蓝光蛋白，可控制集胞藻6803（Synechocystis sp. PCC6803）中的趋光性。PixD的晶体结构显示为十聚体，尽管在溶液中，二聚体和十聚体之间保持着平衡。由于这两种构象体的比例会因光照而改变，因此平衡状态决定了光敏性。然而，目前尚无关于PixD二聚体的结构信息。在此，我们基于对接模拟、诱变和基于质谱的交联分析，报告了二聚体的预测结构。结果表明，PixD C末端对于二聚体偏好和光敏性具有重要作用。
