Pultorak E L, Linder K, Maggi R G, Balakrishnan N, Breitschwerdt E B
Intracellular Pathogens Research Laboratory, Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA.
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
J Comp Pathol. 2015 Jul;153(1):14-21. doi: 10.1016/j.jcpa.2015.04.001. Epub 2015 May 14.
Canine cutaneous histiocytoma (CCH) is a common, benign neoplastic proliferation of histiocytes of Langerhans cell origin that often ulcerate, become secondarily infected and regress spontaneously. Bartonella is a fastidious genus of facultative intracellular pathogens that can be transmitted through arthropod bites and epidermal animal scratches and has been identified previously in the cytoplasm of histiocytes within granulomatous lesions and in skin biopsy samples of inflammatory pustules and papules. Based on the established inflammatory and oncogenic properties of Bartonella, we hypothesized that Bartonella spp. DNA could be amplified from CCH more often than from non-lesional skin and bacteria could be localized within skin tumours using indirect immunofluorescence (IIF). Paraffin wax-embedded surgical biopsy samples from dogs with CCH and non-neoplastic skin adjacent to osteosarcomas (control group selected due to wide surgical margins) were retrieved from the archive of the pathology service of North Carolina State University College of Veterinary Medicine. DNA was extracted and regions of the 16S-23S rRNA intergenic transcribed spacer (ITS) region and the pap31 and gltA genes were amplified by polymerase chain reaction (PCR) using Bartonella-specific primers. IIF was performed using a primary Bartonella henselae monoclonal antibody to localize B. henselae in tissues of PCR-positive dogs. Bartonella vinsonii subsp. berkhoffii was amplified from 1/17 (5.8%) control tissues and B. henselae was amplified from 4/29 (13.8%) CCH tissues. The prevalence of B. vinsonii subsp. berkhoffii (P = 0.37) or B. henselae (P = 0.28) did not vary statistically between study groups. B. henselae could be visualized in 2/4 (50.0%) CCH tissues using IIF. Based on this study, Bartonella spp. are unlikely to cause CCH.
犬皮肤组织细胞瘤（CCH）是一种常见的、起源于朗格汉斯细胞的组织细胞良性肿瘤性增殖，常发生溃疡、继发感染并自发消退。巴尔通体是一类兼性细胞内病原体，属于苛求菌属，可通过节肢动物叮咬和动物表皮抓伤传播，此前已在肉芽肿性病变组织细胞的细胞质以及炎性脓疱和丘疹的皮肤活检样本中被鉴定出来。基于已确定的巴尔通体的炎症和致癌特性，我们推测，与非病变皮肤相比，从CCH中更常能扩增出巴尔通体属DNA，并且使用间接免疫荧光法（IIF）可将细菌定位在皮肤肿瘤内。从北卡罗来纳州立大学兽医学院病理科存档中获取了患有CCH的犬的石蜡包埋手术活检样本以及骨肉瘤旁的非肿瘤性皮肤样本（选择该对照组是因为手术切缘宽）。提取DNA，并使用巴尔通体特异性引物通过聚合酶链反应（PCR）扩增16S - 23S rRNA基因间隔转录区（ITS）以及pap31和gltA基因区域。使用抗汉赛巴尔通体单克隆抗体进行IIF，以在PCR阳性犬的组织中定位汉赛巴尔通体。从1/17（5.8%）的对照组织中扩增出文森巴尔通体伯克霍夫亚种，从4/29（13.8%）的CCH组织中扩增出汉赛巴尔通体。研究组之间文森巴尔通体伯克霍夫亚种（P = 0.37）或汉赛巴尔通体（P = 0.28）的患病率在统计学上没有差异。使用IIF可在2/4（50.0%）的CCH组织中观察到汉赛巴尔通体。基于本研究，巴尔通体属不太可能导致CCH。
