一种用于淋巴因子激活杀伤细胞的新型高产连续细胞培养系统。
A new high-yield continuous cell-culture system for lymphokine-activated killer cells.
作者信息
Noto T, Tokuda Y, Nakamura Y, Suzuki A, Watanabe K, Yamamura M, Tajima T, Mitomi T, Nishijima K
机构信息
Department of Surgery, Tokai University School of Medicine, Kanagawa, Japan.
出版信息
Cancer Immunol Immunother. 1989;30(1):1-4. doi: 10.1007/BF01665023.
Abstract
We performed basic studies on a new high-yield culture system (concentrate rotary tissue-culture system) for application to adoptive immunotherapy with lymphokine-activated killer (LAK) cells. Using this system, we demonstrated that up to 2 x 10(7) peripheral blood mononuclear cells/ml could be cultured in interleukin-2 with a sufficient recovery rate and cytotoxicity in short-term cultures (6 days). This system can also be used to proliferate LAK cells to four times the initial cell number with sufficient cytotoxicity for 14 days of culture. Thus, this system allows activation of sufficient numbers of cells to conduct clinical trials on humans.
摘要
我们对一种新的高产培养系统（浓缩旋转组织培养系统）进行了基础研究，该系统用于淋巴因子激活的杀伤细胞（LAK细胞）的过继免疫治疗。使用该系统，我们证明在白细胞介素-2中，短期培养（6天）时每毫升可培养多达2×10⁷外周血单个核细胞，且具有足够的回收率和细胞毒性。该系统还可用于将LAK细胞增殖至初始细胞数的四倍，在培养14天时具有足够的细胞毒性。因此，该系统能够激活足够数量的细胞以开展人体临床试验。
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本文引用的文献
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