一种新型紧凑且细胞密集的连续培养系统。

A new compact and cell dense continuous culture system.

作者信息

Nakamura Y, Watanabe K, Noto T, Tajima T, Yamamura M

机构信息

Blood Transfusion Service Center, School of Medicine, Tokai University, Kanagawa-ken, Japan.

出版信息

J Immunol Methods. 1989 Mar 10;118(1):31-5. doi: 10.1016/0022-1759(89)90049-5.

DOI:10.1016/0022-1759(89)90049-5

PMID:2926150

Abstract

A new compact and cell dense continuous culture system was developed. The system consisted of a culture bag, which had two compartments separated by a semipermeable membrane, and an external rotator. Cells were cultured in the inner compartment with medium containing serum or the necessary growth factors. The outer compartment was filled with medium without serum or growth factors. The concentration of cells in culture was normally in the order of 10(7). Due to the large number of cells, sufficient carbon dioxide was produced so that use of a CO2 incubator was unnecessary. The medium in the outer part was frequently changed, but no medium changes were necessary in the inner bag. Using this system, a concentrated yield of antibody as well as a large number of cells could be obtained.

摘要

开发了一种新型紧凑且细胞密集的连续培养系统。该系统由一个培养袋和一个外部旋转器组成，培养袋有两个由半透膜隔开的隔室。细胞在内隔室中用含有血清或必要生长因子的培养基培养。外隔室填充不含血清或生长因子的培养基。培养物中的细胞浓度通常为10⁷数量级。由于细胞数量众多，产生了足够的二氧化碳，因此无需使用二氧化碳培养箱。外部的培养基经常更换，但内袋中的培养基无需更换。使用该系统，可以获得高浓度的抗体产量以及大量细胞。